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Product Info Summary
| SKU: | A03471 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-APC1 ANAPC1 Antibody
SKU/Catalog Number
A03471
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APC1 ANAPC1 Antibody (Catalog # A03471). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
APC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APC1 ANAPC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03471)
Host
Rabbit
Contents
APC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
APC1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human APC1. The immunogen is located within amino acids 1810 - 1860 of APC1.
Reactive Species
A03471 is reactive to ANAPC1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
216.5 kDa
Background of ANAPC1
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03471 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APC1 antibody can be used for detection of APC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of APC1 in human brain tissue with APC1 antibody at 5 μg/mL.
Specific Publications For Anti-APC1 ANAPC1 Antibody (A03471)
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