Product Info Summary
SKU: | A07203-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-APEX2 Antibody Picoband™
SKU/Catalog Number
A07203-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-APEX2 Antibody Picoband™ catalog # A07203-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APEX2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07203-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human APEX2 recombinant protein (Position: M1-G500).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07203-1 is reactive to APEX2 in Human
Applications
A07203-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
57 kDa
Calculated molecular weight
57.401kDa
Background of APEX2
APEX2, also called apurinic/apyrimidinic endonuclease like-2, is a member of the apurinic/apyrimidinic (AP) family of endonucleases that initiate the repair of AP sites formed by spontaneous hydrolysis of the N-glycosylic bond, mutagen-induced base release, or damaged-base excision by a DNA repair glycosylase. RT-PCR detected APEX2 expression in HeLa cells, Jurkat cells, and human kidney, brain and fetal brain tissue. The APEX2 gene is mapped to chromosome Xp11.21. APEX2 participates in both nuclear and mitochondrial base excision repair (BER) and it can play a role in processing 3-prime-damaged termini or 3-prime-mismatched nucleotides. Additionally, APEX2 displayed weaker AP site-specific and 3-prime nuclease activities compared to APEX1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry(Fixed), 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of APEX2 using anti-APEX2 antibody (A07203-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX2 antigen affinity purified polyclonal antibody (Catalog # A07203-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APEX2 at approximately 57 kDa. The expected band size for APEX2 is at 57 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of HL-60 cells using anti-APEX2 antibody (A07203-1).
Overlay histogram showing HL-60 cells stained with A07203-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APEX2 Antibody (A07203-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For APEX2 (Source: Uniprot.org, NCBI)
Gene Name
APEX2
Full Name
DNA-(apurinic or apyrimidinic site) lyase 2
Weight
57.401kDa
Superfamily
DNA repair enzymes AP/ExoA family
Alternative Names
AP endonuclease 2; APE2; APEX nuclease (apurinic/apyrimidinic endonuclease) 2; APEX nuclease 2; APEXL2; Apurinic-apyrimidinic endonuclease 2; DNA-(apurinic or apyrimidinic site) lyase 2; DNA-apurinic or apyrimidinic site lyase 2; EC 3.1; EC 4.2.99.18; XTH2 APEX2 APE2, APEXL2, XTH2, ZGRF2 apurinic/apyrimidinic endodeoxyribonuclease 2 DNA-(apurinic or apyrimidinic site) endonuclease 2|AP endonuclease 2|AP endonuclease XTH2|APEX nuclease (apurinic/apyrimidinic endonuclease) 2|DNA-(apurinic or apyrimidinic site) lyase 2|apurinic/apyrimidinic endonuclease-like 2|zinc finger, GRF-type containing 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APEX2, check out the APEX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APEX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APEX2 Antibody Picoband™ (A07203-1)
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No publications found for A07203-1
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