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Product Info Summary
| SKU: | A01841-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-APOL1 Antibody
SKU/Catalog Number
A01841-2
Size
80 µl
Description
Boster Bio Anti-APOL1 Antibody (Catalog # A01841-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-APOL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01841-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This APOL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-171 amino acids from human APOL1.
Reactive Species
A01841-2 is reactive to APOL1 in Human
Calculated molecular weight
44.0 kDa
Background of APOL1
APOL1 is a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01841-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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APOL1-BH3 Domain Antibody western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the APOL1-BH3 Domain antibody detected the APOL1-BH3 Domain protein (arrow).
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APOL1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APOL1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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APOL1 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-APOL1 Antibody (A01841-2)
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