Anti-AQP11 Antibody Picoband™

Product Name

Anti-AQP11 Antibody Picoband™

View all Aquaporin-11 Antibodies

SKU/Catalog Number

PB10044

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AQP11 Antibody Picoband™ catalog # PB10044. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AQP11 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10044)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human AQP11, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10044 is reactive to Aqp11 in Human, Mouse, Rat

Applications

PB10044 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

30 kDa

Calculated molecular weight

30.481kDa

Background of Aquaporin-11

AQP11 has a unique asparagine-proline-alanine (NPA) box distinct from those of other AQPs, suggesting a different pore structure and function. Using Northern blot analysis, they detected highest expression of mouse Aqp11 in testis, followed by liver and kidney. Expression was much weaker in heart, brain, and muscle. Western blot analysis of mouse kidney membrane fractions detected Aqp11 at an apparent molecular mass of 26 kD, lower than the calculated molecular mass of 30 kD. Immunohistochemical analysis localized Aqp11 to mouse renal proximal tubule cells, where it showed a perinuclear distribution. Fluorescence-tagged Aqp11 localized with an endoplasmic reticulum marker.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of AQP11 using anti-AQP11 antibody (PB10044).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP11 antigen affinity purified polyclonal antibody (Catalog # PB10044) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP11 at approximately 30 kDa. The expected band size for AQP11 is at 30 kDa.

Gene/Protein Information For Aqp11 (Source: Uniprot.org, NCBI)

Alternative Names

AQPX1AQP-11; aquaporin 11; aquaporin-11 Aqp11|1700015P13Rik, AI648923, sj, sjds|aquaporin 11|aquaporin-11|AQP-11|CG12251 gene product

For more info on Aqp11, check out the Aqp11 Infographic

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In this infographic, you will see the following information for Aqp11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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