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Product Info Summary
| SKU: | A01600-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATF1 Antibody
SKU/Catalog Number
A01600-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATF1 Antibody (Catalog# A01600-1). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01600-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ATF1. Immunogen sequence location: 176-225
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01600-1 is reactive to ATF1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
29.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01600-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using ATF-1 Polyclonal Antibody diluted at 1:500.
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Western blot analysis of 293T 3T3 HELA KB SH-SY5Y lysis using ATF-1 antibody. Antibody was diluted at 1:500.
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Western blot analysis of lysates from HepG2, COLO205, and HeLa cells, using ATF1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ATF1 Antibody (A01600-1)
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