Anti-Atg4A Monoclonal Antibody

Product Name

Anti-Atg4A Monoclonal Antibody

View all ATG4A Antibodies

SKU/Catalog Number

M06539

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Atg4A Monoclonal Antibody catalog # M06539. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Atg4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06539)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACHB-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06539 is reactive to ATG4A in Human, Mouse, Rat

Applications

M06539 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

45.378kDa

Background of ATG4A

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Atg4A using anti-Atg4A antibody (M06539).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Atg4A antigen affinity purified monoclonal antibody (Catalog # M06539) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Atg4A at approximately 45 kDa. The expected band size for Atg4A is at 45 kDa.

Gene/Protein Information For ATG4A (Source: Uniprot.org, NCBI)

Alternative Names

APG4 autophagy 4 homolog A (S. cerevisiae); Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; Atg4al; Autl2; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase (S. cerevisiae); Autophagin 2; autophagin-2; cysteine endopeptidase; cysteine protease ATG4A; EC 3.4.22; EC 3.4.22.-; hAPG4A ATG4A APG4A, AUTL2 autophagy related 4A cysteine peptidase cysteine protease ATG4A|APG4 autophagy 4 homolog A|ATG4 autophagy related 4 homolog A|AUT-like 2, cysteine endopeptidase|autophagin 2|autophagy-related cysteine endopeptidase 2|autophagy-related protein 4 homolog A|hAPG4A

For more info on ATG4A, check out the ATG4A Infographic

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In this infographic, you will see the following information for ATG4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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