Anti-ATR Antibody

Product Name

Anti-ATR Antibody

View all ATR Antibodies

SKU/Catalog Number

A00262

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ATR Antibody (Catalog # A00262). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-ATR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00262)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00262 is reactive to ATR in Human

Applications

A00262 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

301.367kDa

Background of ATR

Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidyl-inositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4).  ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during S-phase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:15,000 - 1:70,000
WB: 1:1,000 - 1:5,000

Validation Images & Assay Conditions

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Western blot analysis of ATR expression in HELA cell nuclear extract (lane 1), MW markers (Lane M) and negligible staining after pre-incubation of antibody with the immunizing peptide (50 μg peptide for 1 h at room temperature followed by centrifugation) (lane 2). ATR at 301KD was detected using rabbit anti-ATR Antigen Affinity purified polyclonal antibody (Catalog # A00262) at 1:1400. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For ATR (Source: Uniprot.org, NCBI)

Alternative Names

ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR; EC 2.7.11.1; FRAP-related protein 1; FRP1; FRP1FRAP-related protein-1; MEC1; protein kinase ATR; Rad3 related protein; SCKL1; SCKLMEC1, mitosis entry checkpoint 1, homolog; serine/threonine-protein kinase ATR ATR FCTCS, FRP1, MEC1, SCKL, SCKL1 ATR serine/threonine kinase serine/threonine-protein kinase ATR|FRAP-related protein-1|MEC1, mitosis entry checkpoint 1, homolog|ataxia telangiectasia and Rad3-related protein

For more info on ATR, check out the ATR Infographic

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In this infographic, you will see the following information for ATR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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