|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-Aurora A Antibody|
|Description||Rabbit IgG polyclonal antibody for Aurora kinase A(AURKA) detection. Tested with WB in Mouse;Rat.|
|Cite This Product||Anti-Aurora A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1785)|
|Replacement Item||This antibody may replace the following items: sc-109910|sc-14318|sc-14321|sc-25425|sc-293126|sc-398814|sc-514374|sc-56881 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse Aurora A(109-125aa QKTEDTKKRQWTLEDFD), different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with otherproteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Aurora kinase A|
|Molecular Weight||44772 MW|
|Protein Function||Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. .|
|Tissue Specificity||Detected in embryonic neurons in dorsal root ganglia and brain cortex (at protein level). Highly expressed in testis, in about one third of the seminiferous tubules. Expression is restricted to specific spermatocytes nearing completion of prophase, with levels falling off on transition to elongated spermatids. Highly expressed in the ovary, expression in the oocyte starts around the transition to large growing follicle. Abundant expression is seen in the proliferating granulosa and thecal cells of the growing follicle, and in the young corpus luteum. Very weakly expressed in spleen and intestine. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.|
|Subcellular Localization||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. Associates with both the pericentriolar material (PCM) and centrioles. Colocalized with SIRT2 at centrosome (By similarity). Detected at the neurite hillock in developing neurons. .|
|Alternative Names||Aurora kinase A;22.214.171.124;Aurora 2;Aurora family kinase 1;Aurora/IPL1-related kinase 1;ARK-1;Aurora-related kinase 1;Ipl1- and aurora-related kinase 1;Serine/threonine-protein kinase 6;Serine/threonine-protein kinase Ayk1;Serine/threonine-protein kinase aurora-A;Aurka;Aik, Airk, Ark1, Aura, Ayk1, Btak, Iak1, Stk15, Stk6;|
|Research Areas|||cell biology|cell cycle|cell division|spindle| signal transduction|protein phosphorylation|ser / thr kinases|aurora| cancer|tumor biomarkers||
Background for Aurora kinase A
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Aurora A Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Aurora A Antibody Images
Click the images to enlarge.
All lanes: Anti AURKA (PA1785) at 0.5ug/ml
WB: Mouse Ovary Tissue Lysate at 50ug
Predicted bind size: 46KD
Observed bind size: 46KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,