Anti-Aurora A/AURKA Antibody
Rabbit IgG polyclonal antibody for Aurora kinase A(AURKA) detection. Tested with WB in Mouse;Rat.
Anti-Aurora A/AURKA Antibody Info At A Glance
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
|Product Name||Anti-Aurora A/AURKA Antibody
See all Aurora A primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Aurora A/AURKA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Mouse. Aurora A/AURKA information: Molecular Weight: 44772 MW; Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. Associates with both the pericentriolar material (PCM) and centrioles. Colocalized with SIRT2 at centrosome (By similarity). Detected at the neurite hillock in developing neurons; Tissue Specificity: Detected in embryonic neurons in dorsal root ganglia and brain cortex (at protein level). Highly expressed in testis, in about one third of the seminiferous tubules. Expression is restricted to specific spermatocytes nearing completion of prophase, with levels falling off on transition to elongated spermatids. Highly expressed in the ovary, expression in the oocyte starts around the transition to large growing follicle. Abundant expression is seen in the proliferating granulosa and thecal cells of the growing follicle, and in the young corpus luteum. Very weakly expressed in spleen and intestine.|
|Cite This Product||Anti-Aurora A/AURKA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1785)|
|Similar Products From
|Anti-Aurora A/AURKA Antibody may replace the following items: sc 109910|sc 14318|sc 14321|sc 25425|sc 293126|sc 398814|sc 514374|sc 56881.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse Aurora A(109-125aa QKTEDTKKRQWTLEDFD), different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with otherproteins|
Gene/Protein Basic Information For Aurka (Source: Uniprot.org, NCBI)
|NCBI Gene Id||20878|
|Species Of This Entry||Mouse|
|Protein Name||Aurora kinase A|
|Superfamily||protein kinase superfamily|
|Alternative Names||Aurora A|AIK; AIKAurA; ARK1; ARK-1; ARK1EC 18.104.22.168; AURA; AURKA; Aurora A; aurora kinase AhARK1; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora-related kinase 1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; BTAKAURORA2; EC 2.7.11; IPL1-related kinase; serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK15serine/threonine kinase 15; STK6; STK6MGC34538; STK7|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Ubiquitination; Reduction; Dephosphorylation|
|Gene Location||2 H3|2 94.84 cM, on Chromasome 2, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Aurka (Source: Uniprot.org, NCBI)
|Protein Function||Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.|
|Tissue Specificity||Detected in embryonic neurons in dorsal root ganglia and brain cortex (at protein level). Highly expressed in testis, in about one third of the seminiferous tubules. Expression is restricted to specific spermatocytes nearing completion of prophase, with levels falling off on transition to elongated spermatids. Highly expressed in the ovary, expression in the oocyte starts around the transition to large growing follicle. Abundant expression is seen in the proliferating granulosa and thecal cells of the growing follicle, and in the young corpus luteum. Very weakly expressed in spleen and intestine.|
|Research Areas||Breast Cancer; Cancer; Cell Cycle and Replication; Checkpoint signaling; Chromatin Research; Core ESC-Like Genes; MAP Kinase Signaling; Mitotic Regulators; Protein Kinase; Signal Transduction; Stem Cell Markers; Tumor Suppressors|
|Related Diseases||Malignant Neoplasms; Neoplasms; Malignant Neoplasm Of Breast; Carcinoma; Mammary Neoplasms; Cell Transformation, Neoplastic; Aneuploidy; Colorectal Cancer; Adenocarcinoma; Carcinogenesis; Colorectal Neoplasms; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Ovary; Neoplasm Metastasis; Genomic Instability; Ovarian Neoplasm; Malignant Neoplasm Of Pancreas; Malignant Neoplasm Of Prostate; Multiple Myeloma; Leukemia|
|Related Pathways||Cell Cycle; Mitosis; Oocyte Maturation; Chromosome Segregation; Meiosis; Cell Proliferation; Cell Division; Pathogenesis; Cell Death; Cell Growth; Cytokinesis; Spindle Assembly; Metaphase; Localization; Cell Adhesion; Dna Repair; Cell Cycle Arrest; Reverse Transcription; Meiosis I; Embryo Development|
|Background||AURKA(aurora kinase A), also called ARK1, AurA, AIK , AURORA2 ,BTAK, PPP1R47, STK7, STK15,STK6, is a mitotic centrosomal protein kinase. The main role of AURKA in tumor development is in controlling chromosome segregation during mitosis. Aurora A is a member of a family of mitotic serine/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of AURKA occurs during the G2/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Activation of AURKA was independently sufficient to induce rapid ciliary resorption, and AURKA acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of AURKA and HDAC6 reduced regulated disassembly of cilia.|
|Scientific References||PMID: 9245792 by Gopalan G., et al. A novel mammalian, mitotic spindle-associated kinase is related to yeast and fly chromosome segregation regulators.|
PMID: 9205101 by Yanai A., et al. ayk1, a novel mammalian gene related to Drosophila aurora centrosome separation kinase, is specifically expressed during meiosis.
Our Boster Quality Guarantee for Anti-Aurora A/AURKA Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti AURKA (PA1785) at 0.5ug/ml
WB: Mouse Ovary Tissue Lysate at 50ug
Predicted bind size: 46KD
Observed bind size: 46KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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