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Product Info Summary
| SKU: | PA1785 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Aurora A/AURKA Antibody Picoband®
SKU/Catalog Number
PA1785
BA3798-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aurora A/AURKA Antibody catalog # PA1785. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aurora A/AURKA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1785)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse Aurora A, different from the related rat sequence by one amino acid.
Cross-reactivity
No cross-reactivity with otherproteins
Reactive Species
PA1785 is reactive to Aurka in Mouse, Rat
Observed Molecular Weight
46 kDa
Calculated molecular weight
44.8 kDa
Background of Aurka
AURKA (aurora kinase A), also called ARK1, AurA, AIK , AURORA2 ,BTAK, PPP1R47, STK7, STK15,STK6, is a mitotic centrosomal protein kinase. The main role of AURKA in tumor development is in controlling chromosome segregation during mitosis. Aurora A is a member of a family of mitotic serine/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of AURKA occurs during the G2/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Activation of AURKA was independently sufficient to induce rapid ciliary resorption, and AURKA acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of AURKA and HDAC6 reduced regulated disassembly of cilia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1785 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Mouse Ovary Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-AURKA antibody, PA1785, Western blotting
All lanes: Anti AURKA (PA1785) at 0.5ug/ml
WB: Mouse Ovary Tissue Lysate at 50ug
Predicted bind size: 46KD
Observed bind size: 46KD
Specific Publications For Anti-Aurora A/AURKA Antibody Picoband® (PA1785)
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Customer Q&As
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4 Customer Q&As for Anti-Aurora A/AURKA Antibody Picoband®
Question
Will anti-Aurora A/AURKA antibody PA1785 work on bovine WB with blood?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
Our lab technicians have not tested anti-Aurora A/AURKA antibody PA1785 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Aurora A/AURKA antibody PA1785 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-23
Question
We are currently using anti-Aurora A/AURKA antibody PA1785 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
The anti-Aurora A/AURKA antibody (PA1785) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-16
Question
Here is the WB image, lot number and protocol we used for kidney muscle using anti-Aurora A/AURKA antibody PA1785. Please let me know if you require anything else.
D. Jackson
Verified customer
Asked: 2017-07-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-17
Question
I see that the anti-Aurora A/AURKA antibody PA1785 works with WB, what is the protocol used to produce the result images on the product page?
C. Anderson
Verified customer
Asked: 2014-01-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-01-22
