Product Info Summary
SKU: | P00762 |
---|---|
Size: | 100ug |
Reactive Species: | Human, Monkey |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Aurora B pT232 Aurkb Antibody
SKU/Catalog Number
P00762
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Phospho-Aurora B pT232 Aurkb Antibody (Catalog # P00762). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store Phospho Specific Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-Phospho-Aurora B pT232 Aurkb Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00762)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00762 is reactive to AURKB in Human, Monkey
Applications
P00762 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
39.311kDa
Background of Aurora B
Aurora Kinase B (Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Anti-AUROA B pT232 Antibody is useful for researchers interested in gene expression, DNA damage, cytokinesis, and transcription activities.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:30,000
IHC: User optimized
IF Microscopy: User optimized
WB: 1:250 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Aurora B expression in Extract from COS7 cells treated with Nocodazole (lane 1) and extract from COS7 cells treated with Nocodazole and with the phosphopeptide immunogen (lane 2). Aurora B at 39KD was detected using rabbit anti-Aurora B pT232 Antigen Affinity purified polyclonal antibody (Catalog # P00762) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Click image to see more details
AuroraB was detected in paraffin-embedded sections of human intestine pH9 (A) at 20x and 40x using rabbit anti-AuroraB pT232 Antigen Affinity purified polyclonal antibody (Catalog # P00762) at 10 μg/mL. Staining: AuroraB pT232 as precipitated brown signal with hematoxylin purple nuclear counterstain.
Click image to see more details
AuroraB was detected in paraffin-embedded sections of human placenta pH9 (A) at 20x and 40x using rabbit anti-AuroraB pT232 Antigen Affinity purified polyclonal antibody (Catalog # P00762) at 10 μg/mL. Staining: AuroraB pT232 as precipitated brown signal with hematoxylin purple nuclear counterstain.
Protein Target Info & Infographic
Gene/Protein Information For AURKB (Source: Uniprot.org, NCBI)
Gene Name
AURKB
Full Name
Aurora kinase B
Weight
39.311kDa
Superfamily
protein kinase superfamily
Alternative Names
Aik2; AIK2EC 2.7.11.1; AIM1; AIM-1; AIM1Aik2; AIM-1STK-1; ARK2; ARK2ARK-2; AurB; AURKB; aurkb-sv2; Aurora- and IPL1-like midbody-associated protein 1; Aurora B; aurora kinase BSerine/threonine-protein kinase aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora/IPL1-related kinase 2; aurora-1; aurora-B; Aurora-related kinase 2; EC 2.7.11; IPL1; serine/threonine kinase 12; serine/threonine-protein kinase 12; STK12; STK12aurkb-sv1; STK5 AURKB AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2 aurora kinase B aurora kinase B|aurora kinase B-Sv1|aurora kinase B-Sv2|aurora- and Ipl1-like midbody-associated protein 1|aurora-1|aurora-B|aurora-related kinase 2|aurora/IPL1-related kinase 2|protein phosphatase 1, regulatory subunit 48|serine/threonine kinase 12|serine/threonine-protein kinase 12|serine/threonine-protein kinase 5|serine/threonine-protein kinase aurora-B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AURKB, check out the AURKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AURKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Aurora B pT232 Aurkb Antibody (P00762)
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No publications found for P00762
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-Aurora B pT232 Aurkb Antibody
Question
I am interested in to test anti-Phospho-Aurora B pT232 antibody P00762 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Phospho-Aurora B pT232 antibody P00762 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The products we sell, including anti-Phospho-Aurora B pT232 antibody P00762, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-17
Question
We are currently using anti-Phospho-Aurora B pT232 antibody P00762 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-Phospho-Aurora B pT232 antibody (P00762) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27
Question
I see that the anti-Phospho-Aurora B pT232 antibody P00762 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-15
Question
I am looking for using your anti-Phospho-Aurora B pT232 antibody for spindle organization studies. Has this antibody been tested with western blotting on cos7 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Thanks for your inquiry. This P00762 anti-Phospho-Aurora B pT232 antibody is validated on cos7 cells. It is guaranteed to work for IF, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-20
Question
Does anti-Phospho-Aurora B pT232 antibody P00762 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
According to the expression profile of cervix carcinoma, AURKB is highly expressed in cervix carcinoma. So, it is likely that anti-Phospho-Aurora B pT232 antibody P00762 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-10-29
Question
I was wanting to use your anti-Phospho-Aurora B pT232 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
It shows on the product datasheet, P00762 anti-Phospho-Aurora B pT232 antibody has been validated for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-06
Question
Is there a BSA free version of anti-Phospho-Aurora B pT232 antibody P00762 available?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Aurora B pT232 antibody P00762 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-17
Question
We were well pleased with the WB result of your anti-Phospho-Aurora B pT232 antibody. However we have observed positive staining in cervix carcinoma centromere. using this antibody. Is that expected? Could you tell me where is AURKB supposed to be expressed?
R. Li
Verified customer
Asked: 2019-06-13
Answer
According to literature, cervix carcinoma does express AURKB. Generally AURKB expresses in nucleus. chromosome. chromosome, centromere. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-13
Question
Is this P00762 anti-Phospho-Aurora B pT232 antibody reactive to the isotypes of AURKB?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
The immunogen of P00762 anti-Phospho-Aurora B pT232 antibody is A synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Phospho-Aurora B pT232 antibody P00762. Let me know if you need anything else.
R. Bhatt
Verified customer
Asked: 2018-12-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-27
Question
Is a blocking peptide available for product anti-Phospho-Aurora B pT232 antibody (P00762)?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
We do provide the blocking peptide for product anti-Phospho-Aurora B pT232 antibody (P00762). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-25
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-Phospho-Aurora B pT232 antibody P00762. Please let me know if you require anything else.
A. Anderson
Verified customer
Asked: 2017-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-31
Question
Does P00762 anti-Phospho-Aurora B pT232 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
You can see on the product datasheet, P00762 anti-Phospho-Aurora B pT232 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-31
Question
We have observed staining in human cervix carcinoma. Are there any suggestions? Is anti-Phospho-Aurora B pT232 antibody supposed to stain cervix carcinoma positively?
H. Collins
Verified customer
Asked: 2017-05-25
Answer
Based on literature cervix carcinoma does express AURKB. Based on Uniprot.org, AURKB is expressed in oocyte, liver spleen, lung, lymph muscle, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-25
Question
We purchased anti-Phospho-Aurora B pT232 antibody for WB on cervix carcinoma erythroleukemia in a previous experiment. I am using human, and We are going to use the antibody for IHC next. Our lab want to know about examining cervix carcinoma erythroleukemia as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
J. Li
Verified customer
Asked: 2015-09-14
Answer
I looked at the website and datasheets of our anti-Phospho-Aurora B pT232 antibody and it appears that P00762 has been tested on human in both WB and IHC. Thus P00762 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-09-14
Question
I have a question about product P00762, anti-Phospho-Aurora B pT232 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Roberts
Verified customer
Asked: 2015-05-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00762 anti-Phospho-Aurora B pT232 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-05-21