Click image to see more details
Product Info Summary
| SKU: | A00075 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-B-raf Antibody
SKU/Catalog Number
A00075
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-B-raf Antibody (Catalog # A00075). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
B-raf antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-B-raf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00075)
Host
Rabbit
Contents
B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
Anti-B-raf antibody was raised against a peptide corresponding to 19 amino acids near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody will not cross-react with C-raf.
Reactive Species
A00075 is reactive to BRAF in Human, Mouse
Applications
A00075 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
84.437kDa
Background of B-Raf
B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western Blot Validation in Human Brain Tissue
Loading: 15 μg of lysates per lane. Antibodies: B-raf A00075, (A:1 μg/mL; B:2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: B-raf, (0.5 μg/mL), B-raf A00075, (0.5 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken and goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Immunohistochemistry Validation of B-raf in Human Small Intestine Tissue
Immunohistochemical analysis of paraffin-embedded Human Small Intestine Tissue using anti-B-raf antibody (A00075) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Click image to see more details
Immunofluorescence Validation of B-raf in Human Small Intestine Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Small Intestine Tissue labeling B-raf with A00075 at 20 μg/mL
Protein Target Info & Infographic
Gene/Protein Information For BRAF (Source: Uniprot.org, NCBI)
Gene Name
BRAF
Full Name
Serine/threonine-protein kinase B-raf
Weight
84.437kDa
Superfamily
protein kinase superfamily
Alternative Names
94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109 BRAF B-RAF1, B-raf1, NS7, RAFB1, BRAF B-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase B-raf|94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|B-Raf serine/threonine-protein|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRAF, check out the BRAF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B-raf Antibody (A00075)
Hello CJ!
No publications found for A00075
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-B-raf Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-B-raf Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
