Anti-Bach2 Antibody

Product Name

Anti-Bach2 Antibody

View all Bach2 Antibodies

SKU/Catalog Number

A04096

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Bach2 Antibody (Catalog # A04096). Tested in ELISA, IP, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Bach2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04096)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04096 is reactive to Bach2 in Mouse

Applications

A04096 is guaranteed for ELISA, IP, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

91.836kDa

Background of Bach2

This antibody is suitable for Cancer and DNA Damage & Repair research. During an immune response to infectious diseases and cancer, B-cells undergo activation in response to antigen, and differentiate either into short-lived plasma cells, whose numbers contract following withdrawal of the antigenic stimulus, or into memory B-cells. The formation of B-cell memory is the major goal of vaccination, since memory B-cells are able to produce antibodies specific to infections and cancer that can last for the lifetime of the host. Recently it has been shown that the formation of long-lived memory B-cells is dependent upon the transcription factor Bach2, since knockout mice lacking the gene fail to generate class-switched IgG antibody responses and all B-cells undergo default plasma cell differentiation upon antigenic stimulation. While the mechanism by which Bach2 controls B-cell memory fate has been elucidated (it binds and represses the promoter of the gene that encodes the plasma cell master regulatory transcription factor Blimp-1), the upstream mechanism by which the function of Bach2 is regulated is unknown. There is evidence suggesting that Bach2 is phosphorylated in B-cells following stimulation and suspect that this modification may allow the B-cell signaling apparatus to control Bach2 activity and therefore memory fate decisions. Anti-Bach2 is ideal for researchers interested in Cancer and DNA Damage & Repair research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5000
IP: 1 µg/mL
WB: 1 µg/mL

Validation Images & Assay Conditions

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Western blot analysis of Bach2 expression in 293T cell lysates overexpressing Bach2-Flag (lane 1) and 293T cell lysates (lane 2). Bach2 at 130KD was detected using rabbit anti-Bach2 Antigen Affinity purified polyclonal antibody (Catalog # A04096) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For Bach2 (Source: Uniprot.org, NCBI)

Alternative Names

BTB and CNC homolog 2; BTB and CNC homology 1, basic leucine zipper transcription factor 2; transcription regulator protein BACH2 Bach2|E030004N02Rik|BTB and CNC homology, basic leucine zipper transcription factor 2|transcription regulator protein BACH2|BTB and CNC homolog 2|BTB and CNC homology 2|Transcription regulator protein BACH2 (BTB and CNC homolog 2)

For more info on Bach2, check out the Bach2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Bach2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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