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Product Info Summary
| SKU: | A03520 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BAD Antibody
SKU/Catalog Number
A03520
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BAD Antibody (Catalog # A03520). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BAD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BAD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03520)
Host
Rabbit
Contents
BAD Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad. The immunogen is located within amino acids 90 - 140 of BAD.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
LRRC8A antibody is human, mouse and rat reactive.
Reactive Species
A03520 is reactive to BAD in Human, Mouse, Rat
Applications
A03520 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
18.392kDa
Background of BAD
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival, whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bad antibody can be used for detection of Bad by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5
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Immunohistochemical staining of rat thymus using Bad at 2 μg/mL.
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Immunofluorescence of BAD in Rat Thymus cells with BAD antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BAD (Source: Uniprot.org, NCBI)
Gene Name
BAD
Full Name
Bcl2-associated agonist of cell death
Weight
18.392kDa
Superfamily
Bcl-2 family
Alternative Names
Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter BAD BBC2, BCL2L8 BCL2 associated agonist of cell death bcl2-associated agonist of cell death|BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein|bcl-2-binding component 6|bcl-2-like protein 8|bcl-XL/Bcl-2-associated death promoter|bcl2 antagonist of cell death|bcl2-L-8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAD, check out the BAD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BAD Antibody (A03520)
Hello CJ!
A03520 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xie H,Wang H,Wu Q,Peng J,Huang H,Wang Y,Huang M,Jiang W,Yang Y,Zhang X,Zhang J,Zhu Q. Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury. J Inflamm Res. 2021 Mar 9;14:761-776.doi:10.2147/JIR. S301104.PMID:33727850;PMCID: PMC7955787.
Species: Human,Mouse
A03520 usage in article: APP:WB, SAMPLE:GLOMERULI TISSUE, DILUTION:NA
Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
A03520 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA
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