Product Info Summary
SKU: | M03520 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bad Rabbit Monoclonal Antibody
SKU/Catalog Number
M03520
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Bad Rabbit Monoclonal Antibody catalog # M03520. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bad Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03520)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CHC-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bad
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03520 is reactive to BAD in Human, Mouse, Rat
Applications
M03520 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
18.392kDa
Background of BAD
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Bad Antibody.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Hela cells, using Bad Antibody .
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Western blot analysis of BAD expression in Hela cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For BAD (Source: Uniprot.org, NCBI)
Gene Name
BAD
Full Name
Bcl2-associated agonist of cell death
Weight
18.392kDa
Superfamily
Bcl-2 family
Alternative Names
Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter BAD BBC2, BCL2L8 BCL2 associated agonist of cell death bcl2-associated agonist of cell death|BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein|bcl-2-binding component 6|bcl-2-like protein 8|bcl-XL/Bcl-2-associated death promoter|bcl2 antagonist of cell death|bcl2-L-8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAD, check out the BAD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bad Rabbit Monoclonal Antibody (M03520)
Hello CJ!
M03520 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xie H,Wang H,Wu Q,Peng J,Huang H,Wang Y,Huang M,Jiang W,Yang Y,Zhang X,Zhang J,Zhu Q. Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury. J Inflamm Res. 2021 Mar 9;14:761-776.doi:10.2147/JIR. S301104.PMID:33727850;PMCID: PMC7955787.
Species: Human,Mouse
M03520 usage in article: APP:WB, SAMPLE:GLOMERULI TISSUE, DILUTION:NA
Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
M03520 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
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