Product Info Summary
SKU: | A05089-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-BCAT1 Antibody Picoband™
SKU/Catalog Number
A05089-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BCAT1 Antibody Picoband™ catalog # A05089-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-BCAT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05089-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BCAT1 recombinant protein (Position: L88-S386).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05089-2 is reactive to BCAT1 in Human, Mouse, Rat
Applications
A05089-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.966kDa
Background of BCAT1
BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BCAT1 using anti-BCAT1 antibody (A05089-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat pancreas tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT1 antigen affinity purified polyclonal antibody (Catalog # A05089-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAT1 at approximately 43 kDa. The expected band size for BCAT1 is at 43 kDa.
Click image to see more details
Figure 2. IHC analysis of BCAT1 using anti-BCAT1 antibody (A05089-2).
BCAT1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody (A05089-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of BCAT1 using anti-BCAT1 antibody (A05089-2).
BCAT1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody (A05089-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of BCAT1 using anti-BCAT1 antibody (A05089-2).
BCAT1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody (A05089-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of U937 cells using anti-BCAT1 antibody (A05089-2).
Overlay histogram showing U937 cells stained with A05089-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCAT1 Antibody (A05089-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For BCAT1 (Source: Uniprot.org, NCBI)
Gene Name
BCAT1
Full Name
Branched-chain-amino-acid aminotransferase, cytosolic
Weight
42.966kDa
Superfamily
class-IV pyridoxal-phosphate-dependent aminotransferase family
Alternative Names
BCAT(c); BCAT1; BCAT-1; BCATC; BCT1; BCT1PNAS121; branched chain amino-acid transaminase 1, cytosolic; branched chain aminotransferase 1, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; DKFZp686E12175; EC 2.6.1.42; ECA39; MECA39; placental protein 18; PNAS121; PP18; Protein ECA39 BCAT1 BCATC, BCT1, ECA39, MECA39, PNAS121, PP18 branched chain amino acid transaminase 1 branched-chain-amino-acid aminotransferase, cytosolic|branched chain amino-acid transaminase 1, cytosolic|branched chain aminotransferase 1, cytosolic|placental protein 18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCAT1, check out the BCAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BCAT1 Antibody Picoband™ (A05089-2)
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