Anti-Bcl-2 Antibody (Monoclonal, 35B51)

Product Name

Anti-Bcl-2 Antibody (Monoclonal, 35B51)

View all Bcl2 Antibodies

SKU/Catalog Number

M00040-7
BM0200 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bcl-2 Antibody (Monoclonal, 35B51) catalog # M00040-7. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bcl-2 Antibody (Monoclonal, 35B51) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00040-7)

Host

Mouse

Contents

Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.

Clonality

Monoclonal

Clone Number

35B51

Isotype

Mouse IgG1

Immunogen

Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

M00040-7 is reactive to Bcl2 in Human

Calculated molecular weight

26.6 kDa

Background of Bcl2

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00040-7 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, -
Western blot, 1-2μg/ml, Human

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information Bcl2

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