Apoptosis regulator Bcl-2 Antibodies

AND BCL2 ELISA kits, Apoptosis regulator Bcl-2 Proteins

Many biological assays use antibodies to detect Apoptosis regulator Bcl-2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Apoptosis regulator Bcl-2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Apoptosis regulator Bcl-2 antibodies...

We validate the specificity of these antibodies to Apoptosis regulator Bcl-2 by testing them on tissues known to express BCL2 positively and negatively. Browse below to find the BCL2 antibody that suites your experiment. We have 93 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Apoptosis regulator Bcl-2? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the BCL2 Western Blot Guide

BCL2 Infographic by Boster Bio

All BCL2 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-Bcl-2/BCL2 Antibody Picoband®

158 Citations

Western blot analysis of BCL2 using anti-BCL2 antibody (A00040-2)

IF analysis of BCL2 and Tubulin alpha using anti-BCL2 antibody (A00040-2) and anti-Tubulin alpha antibody (M03989-3)

Flow Cytometry analysis of U20S cells using anti-BCL2 antibody (A00040-2)

+14

Human

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A00040-2
100 μg/vial

Anti-Beclin 1/BECN1 Antibody Picoband®

44 Citations

Western blot analysis of Beclin 1 using anti-Beclin 1 antibody (PB9076)

Flow Cytometry analysis of Raji cells using anti-Beclin 1 antibody (PB9076)

Immunoprecipitating Beclin 1/BECN1 in MCF-7 whole cell lysate

Human, Mouse, Rat

Flow Cytometry, IP, IF, ICC, WB

$370
Cat # PB9076
100 μg/vial

Anti-Bcl-2 Rabbit Monoclonal Antibody

22 Citations

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature

+14

Human, Mouse

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M00040-2
100 μl

Anti-BNIP3L/Nix Rabbit Monoclonal Antibody

4 Citations

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature

Western blot analysis of BNIP3L expression in K562 cell lysate

IHC analysis of BNIP3L using anti-BNIP3L antibody (M03107)

+18

Human, Mouse, Rat

IHC, WB

$370
Cat # M03107
100 μl

Anti-BNIP3 Antibody Picoband®

5 Citations

Down-regulation of miR-145 and up-regulation of BNIP3 expression in gliomas

miR-145 inhibits mRNA and protein expression of BNIP3

Human, Mouse, Rat

$370
Cat # A01469
100 μg/vial

Anti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2)

3 Citations

IHC analysis of BAK1 using anti-BAK1 antibody (M01163-1)

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M01163-1
100 μg/vial

Anti-Bad Rabbit Monoclonal Antibody

12 Citations

Immunofluorescent analysis of Hela cells, using Bad Antibody

Western blot analysis of BAD using anti-BAD antibody (M03520)

IHC analysis of BAD using anti-BAD antibody (M03520)

Human, Mouse, Rat

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M03520
100 μl

Anti-Bcl-XS/BCL2L1 Antibody Picoband®

6 Citations

Anti-Bcl-XS antibody, PA1232, IHC(P)IHC(P): Human Colon Cancer Tissue

IHC analysis of Bcl-XS/BCL2L1 using anti-Bcl-XS/BCL2L1 antibody (PA1232)

Western blot analysis of Bcl-XS/BCL2L1 using anti-Bcl-XS/BCL2L1 antibody (PA1232)

Human, Mouse, Rat

IHC, WB

$370
Cat # PA1232
100 μg/vial

Anti-PKM2 Antibody Picoband® (monoclonal, 11I4C3)

3 Citations

Western blot analysis of PKM2 using anti-PKM2 antibody (M01173-1)

IHC analysis of PKM2 using anti-PKM2 antibody (M01173-1)

Human

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M01173-1
100 μg/vial

Anti-Bcl-XL/BCL2L1 Antibody Picoband®

3 Citations

Western blot analysis of Bcl-XL using anti-Bcl-XL antibody (A00181)

Flow Cytometry analysis of Jurkat cells using anti-Bcl-XL antibody (A00181)

IHC analysis of Bcl-XL using anti-Bcl-XL antibody (A00181)

Human, Mouse, Rat

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # A00181
100 μg/vial

Anti-EIF4EBP1 Antibody Picoband®

3 Citations

Western blot analysis of EIF4EBP1 using anti-EIF4EBP1 antibody (A00968-1)

IHC analysis of EIF4EBP1 using anti-EIF4EBP1 antibody (A00968-1)

Flow Cytometry analysis of A431 cells using anti-EIF4EBP1 antibody (A00968-1)

Human, Mouse, Rat

Flow Cytometry, IHC, WB

$370
Cat # A00968-1
100 μg/vial

Anti-Beclin 1/BECN1 Antibody Picoband®

9 Citations

Anti-Beclin 1 antibody, PA1847, Western blottingLane 1: HELA Cell LysateLane 2: SW620 Cell LysateLane 3: PANC Cell Lysate

Human, Mouse, Rat

$370
Cat # PA1847
100 μg/vial

Anti-HGS Antibody Picoband® (monoclonal, 4B7E2)

Western blot analysis of HGS using anti-HGS antibody (M01174-2)

IHC analysis of HGS using anti-HGS antibody (M01174-2)

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M01174-2
100 μg/vial

Anti-Bim/BCL2L11 Antibody Picoband®

1 Citations

Western blot analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4)

IHC analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4)

Human, Mouse

Flow Cytometry, IHC, WB

$370
Cat # A01552-4
100 μg/vial

Anti-HGS Antibody Picoband® (monoclonal, 6C2E1)

Western blot analysis of HGS using anti-HGS antibody (M00179-1)

IHC analysis of HGS using anti-HGS antibody (M01174-1)

Human, Mouse, Rat

Flow Cytometry, IHC, WB

$370
Cat # M01174-1
100 μg/vial

Anti-BNIP3 Rabbit Monoclonal Antibody

3 Citations

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution

Human, Mouse, Rat

IF, IHC, ICC, WB

$370
Cat # M01469
100 μl

Human Total Bcl-2 ELISA Kit PicoKine®

Human

ELISA

$499
Cat # EK2385
96 wells/kit, with removable strips.

Anti-Bcl-X/BCL2L1 Antibody Picoband®

4 Citations

Western blot analysis of Bcl-X using anti-Bcl-X antibody (PB9917)

Flow Cytometry analysis of PC-3 cells using anti-Bcl-X antibody (PB9917)

Flow Cytometry analysis of A549 cells using anti-Bcl-X antibody (PB9917)

Human

Flow Cytometry, IHC, ICC, WB

$370
Cat # PB9917
100 μg/vial

Anti-Bcl-2 Rabbit Monoclonal Antibody

2 Citations

Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:50 dilution

Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution

Human

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M00040-1
100 μl

Anti-Bim BCL2L11 Rabbit Monoclonal Antibody

1 Citations

Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:50 dilution

Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution

Human, Mouse, Rat

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M01552
100 μl

Anti-BAT3/BAG6 Antibody Picoband®

Western blot analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1)

IHC analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1)

IF analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1) and anti-Beta Tubulin antibody (M01857-3)

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A00967-1
100 μg/vial

Anti-Bcl-XL BCL2L1 Rabbit Monoclonal Antibody

1 Citations

Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:400 dilution

Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody at 1:400 dilution

Immunofluorescent analysis using the Antibody at 1:50 dilution

Human, Mouse, Rat

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M00181-1
100 μl

Anti-Bcl-2-like protein 2 Bcl2L2 Antibody Picoband®

IHC analysis of BCL2L2 using anti-BCL2L2 antibody (PA1426-1)

Flow Cytometry analysis of PC-3 cells using anti-BCL2L2 antibody (PA1426-1)

Western blot analysis of Bcl2L2 using anti-Bcl2L2 antibody (PA1426-1)

Human, Mouse, Rat

Flow Cytometry, IHC, ICC, WB

$370
Cat # PA1426-1
100 μg/vial

Anti-BCLG Rabbit Monoclonal Antibody

Western blot analysis of BCL2L14 using anti-BCL2L14 antibody (M09347-1)

IHC analysis of BCL2L14 using anti-BCL2L14 antibody (M09347-1)

Human, Mouse, Rat

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M09347-1
100 μl/vial

Anti-Bim/Bcl2l11 Antibody Picoband®

Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5)

Flow Cytometry analysis of C2C12 cells using anti-Bim/Bcl2l11 antibody (A01552-5)

Mouse

ELISA, Flow Cytometry, WB

$370
Cat # A01552-5
100 µg/vial

Anti-Bcl2 alpha Antibody Picoband®

Human

ELISA, WB

$370
Cat # RP1003
100 μg/vial

Anti-Bcl2A1 antibody, PA2102, Western blottingAll lanes: Anti Bcl2A1(PA2102) at 0

Human

$370
Cat # PA2102
100 μg/vial

Anti-Bcl-XL Rabbit Monoclonal Antibody

Western blot analysis of Bcl-XL expression in (1) Ramos cell lysate; (2) K562 cell lysate

Human, Mouse, Rat

IP, IHC, WB

$370
Cat # M00181-3
100 µl/vial

Anti-BCL2L15 Rabbit Monoclonal Antibody

Western blot analysis of BCL2L15 in His-tagged BCL2L15 cell lysate

Human

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M14383
100 µl/vial

Anti-BCL2A1/Bcl 2 Related Protein A1 Rabbit Monoclonal Antibody

Western blot analysis of BCL2A1 using anti-BCL2A1 antibody (M03850)

Human, Mouse, Rat

Flow Cytometry, IHC, WB

$370
Cat # M03850
100 μl

Anti-NFYA Rabbit Monoclonal Antibody

Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution

Western blot analysis of NFYA using anti-NFYA antibody (M03524)

Human, Mouse, Rat

Flow Cytometry, IP, IF, ICC, WB

$370
Cat # M03524
100 μl/vial

Anti-HLA DMB Rabbit Monoclonal Antibody

Western blot analysis of HLA DMB expression in Raji cell lysate

Human

Flow Cytometry, WB

$370
Cat # M03526
100 μl/vial

Anti-gamma Synuclein Rabbit Monoclonal Antibody

Western blot analysis of gamma Synuclein expression in human fetal brain lysate

Human, Rat

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M03523
100 μl

Anti-Laminin gamma 1 Rabbit Monoclonal Antibody

Western blot analysis of Laminin gamma 1 expression in A431 cell lysate

Human

Flow Cytometry, WB

$370
Cat # M03522-1
100 μl/vial

Anti-ESRRG Rabbit Monoclonal Antibody

Western blot analysis of ESRRG using anti-ESRRG antibody (M01470-2)

Human, Mouse, Rat

$370
Cat # M01470-2
100 μl/vial

Anti-BCL2L12 Rabbit Monoclonal Antibody

Western blot analysis of BCL2L12 expression in MCF7 cell lysate

Human

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M06508
100 μl/vial

Anti-Beclin-1 BECN1 Antibody

Immunofluorescence Validation of Beclin-1 in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Beclin-1 with A00327-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue)

WB Validation in Human Cell Lines
Loading: 15 μg of lysate
Antibodies: Beclin-1 A00327-1, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST

WB Validation in Mouse Tissues
Loading: 15 μg of lysate
Antibodies: Beclin-1 A00327-1 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST

+10

Human, Mouse, Rat

ELISA, IF, IHC-P, ICC, WB

$481
Cat # A00327-1
0.1 mg

Anti-Bim BCL2L11 Antibody

Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane

Western Blot Validation in Human Tissue
Loading: 15 μg of lysates per lane

Human, Mouse

ELISA, IF, ICC, WB

$481
Cat # A01552
0.1 mg

Anti-PUMA BBC3 Antibody

Western Blot Validation of PUMA in K562 Cells
Loading: 15 μg of lysates per lane

Immunofluorescence Validation of PUMA in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with A04899-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue)

Human, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A04899-1
0.1 mg

Anti-PUMA BBC3 Antibody

Western Blot Validation of PUMA in K562 and 3T3/NIH Cells
Loading: 15 μg of lysates per lane

Validation with PUMA siRNA Knockdown in 293 Cells
293 cells were transfected with control siRNAs (lane 1) or PUMA siRNAs (lane 2)
Loading: 15 μg of 293 whole cell lysates per lane

Human, Mouse

ELISA, IF, ICC, WB

$481
Cat # A04899
0.1 mg

Anti-Phospho-Bcl-2 (T56) Antibody

Western Blot analysis of various cells using Phospho-Bcl-2 (T56) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BCL-2 (Phospho-Thr56) Antibody

Human, Mouse, Rat

ELISA, IP, IF, IHC, WB

$380
Cat # A00040T56-1
100ul

Anti-Bcl-w Antibody

Western Blot analysis of various cells using Bcl-w Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from COLO cells, using BCLW Antibody

Immunohistochemical analysis of paraffin-embedded Human colon cancer

Human, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A33957
100ul

Anti-Bcl-G BCL2L14 Antibody

Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2

Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/mL

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 μg/mL

Human, Mouse, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A09347
0.1 mg

Anti-Bcl-rambo BCL2L13 Antibody

Western blot analysis of Bcl-rambo in K562 cell lysate with Bcl-rambo antibody at (A) 2 and (B) 4 μg/mL

Immunohistochemistry of Bcl-rambo in human heart tissue with Bcl-rambo antibody at 10 μg/mL

Immunofluorescence of Bcl-rambo in Human Heart cells with Bcl-rambo antibody at 20 μg/mL

Human, Mouse, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A06151
0.1 mg

Anti-BAD Antibody

Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0

Immunohistochemical staining of rat thymus using Bad at 2 μg/mL

Immunofluorescence of BAD in Rat Thymus cells with BAD antibody at 10 μg/mL

Human, Mouse, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A03520
0.1 mg

Anti-Bim EL BCL2L11 Antibody

Western blot analysis of Bim in K562 cell lysate with Bim EL antibody at (A) 1 and (B) 2 μg/mL

Immunocytochemistry of BIM EL inK562 cells with BIM EL antibody at 2

Immunofluorescence of BIM EL in K562 cells with BIM EL antibody at 2

Human, Mouse, Rat

ELISA, IF, ICC, WB

$481
Cat # A01552-2
0.1 mg

Anti-Mcl-1 Antibody

Western blot analysis of Mcl-1 in Raji cell lysate with Mcl-1 antibody at 0

Immunocytochemistry staining of Raji cells using Mcl-1 antibody at 2 μg/mL

Immunofluorescence of Mcl-1 in Raji cells with Mcl-1 antibody at 10 μg/mL

Human, Mouse, Rat

ELISA, IF, ICC, WB

$481
Cat # A00712
0.1 mg

Anti-Beclin-1 BECN1 Antibody

Western blot analysis of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0

Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 μg/mL

Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 μg/mL

Human, Mouse

ELISA, IF, ICC, WB

$481
Cat # A00327
0.1 mg

Anti-SODD BAG4 Antibody

Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no

Immunocytochemistry of SODD in HeLa cells with SODD antibody at 5 μg/mL

Immunofluorescence of SODD in Hela cells with SODD antibody at 20 μg/mL

Human, Mouse, Rat

ELISA, IF, ICC, WB

$481
Cat # A06722
0.1 mg

Anti-Bnip3L Antibody

Western blot analysis of Bnip3L in K562 whole cell lysate in (A) the absence, or (B) presence of immunogenic peptide with Bnip3L antibody at 1 μg/mL

Human

ELISA, IF, IHC-P, WB

$481
Cat # A03107
0.1 mg

Anti-Bcl-2-modifying factor Bmf Antibody

Western blot analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 μg/ml

Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 μg/mL

Immunofluorescence of Bmf in HeLa cells with Bmf antibody at 5 μg/mL

Human, Mouse

ELISA, ICC, WB

$481
Cat # A02432-1
0.1 mg

Anti-Bcl-2 (phospho-S70) Antibody

Western blot (WB) analysis of Bcl-2 (phospho-S70) polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:HEK293T treated with UV for 1h whole cell lysate(40ug)
Lane3:HEK293T treated with UV for 6h whole cell lysate(40ug)
Lane4:HEK293T treated with UV for 24h whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Bcl2 (S70) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IP, IHC, WB

$482
Cat # A00040S70-1
100ul

Anti-Bad (phospho-S136) Antibody

Western blot (WB) analysis of Bad (phospho-S136) polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:HEK293T treated with UV for 1h whole cell lysate(40ug)
Lane3:HEK293T treated with UV for 4h whole cell lysate(40ug)
Lane4:HEK293T treated with UV for 16h whole cell lysate(40ug)
Lane5:HEK293T treated with UV for 24h whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Bad (S136) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC, WB

$482
Cat # A03520S136
100ul

Anti-Bcl-2-modifying factor Bmf Antibody

Western blot analysis of Bmf expression in HepG2 cell lysate with Bmf antibody at (A) 2

Human

ELISA, IF, IHC-P, WB

$481
Cat # A02432
0.1 mg

Anti-Bcl-X (S56) BCL2L1 Antibody

Western blot (WB) analysis of Bcl-X (S56) polyclonal antibody at 1:500 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:Hela treated with H2O2 (100nmol/ml, 25min, phostop) whole cell lysate (40ug)
Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase) whole cell lysate (40ug)

Immunohistochemistry (IHC) analyzes of Bcl-X (S56) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC, WB

$405
Cat # A00181S56
100ul

Anti-Bcl-2 (phospho-T69) Antibody

Immunohistochemistry (IHC) analyzes of p-Bcl-2 (T69) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50

Human, Mouse, Rat

IHC

$482
Cat # A00040T69
100ul

Anti-Bcl-2 (phospho-T56) Antibody

Immunohistochemistry (IHC) analyzes of p-Bcl2 (T56) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IP, IF, IHC

$482
Cat # A00040T56
100ul

Anti-Bad (phospho-S91) Antibody

Immunohistochemistry (IHC) analyzes of p-Bad (S91) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC

$482
Cat # A03520S91
100ul

Anti-Bad (phospho-S155) Antibody

Immunohistochemistry (IHC) analyzes of p-Bad (S155) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IF, IHC

$482
Cat # A03520S155
100ul

Anti-Bad (phospho-S112) Antibody

Immunohistochemistry (IHC) analyzes of p-Bad (S112) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC

$482
Cat # A03520S112
100ul

Anti-BNIP-3 (E96) Antibody

Immunohistochemistry (IHC) analyzes of BNIP-3 (E96) pAb in paraffin-embedded human brain tissue

Human, Mouse, Rat

IHC

$405
Cat # A01469-1
100ul

Anti-Bcl-X (phospho-T47) BCL2L1 Antibody

Immunohistochemistry (IHC) analyzes of p-Bcl-X (T47) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC

$482
Cat # A00181T47
100ul

Anti-Bcl-X (S43) BCL2L1 Antibody

Immunohistochemistry (IHC) analyzes of Bcl-X (S43) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IF, IHC

$405
Cat # A00181S43
100ul

Anti-Bcl-2 Monoclonal Antibody

1 Citations

Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by siRNA1 (F:GGAUGACUGAGUACCUGAATT, R:UUCAGGUACUCAGUCAUCCTT) siRNA2 (F:GUGAUGAAGUACAUCCAUUAU, R:AUAAUGGAUGUACUUCAUCAC), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour

Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-BCL-2 antibody

Human, Mouse, Rat

ELISA, IF, WB

$380
Cat # M00040-3
100ul

BCL-2 Colorimetric Cell-Based ELISA Kit

1 Citations

Western blot analysis of extracts from MCF7 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1055
1 kit, containing one 96-well plate and all necessary reagents

BAD (Phospho-Ser136) Colorimetric Cell-Based ELISA Kit

1 Citations

Western blot analysis of extracts from NIH-3T3 cells treated with Forskolin

Human, Mouse, Rat

ELISA

$540
Cat # EKC2359
1 kit, containing two 96-well plates and all necessary reagents

Anti-Bcl-2 Rabbit Monoclonal Antibody

Human spleen was stained with anti-Bcl-2 rabbit antibody

Rat spleen was stained with anti-Bcl-2 rabbit antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bcl-2 antibody

Human, Mouse, Rat

ELISA, IP, IF, IHC, WB

$146
Cat # M00040-4
20μL, 40μL, 100μL, 200μL

Anti-Bcl-XL Rabbit Monoclonal Antibody

Human colon carcinoma was stained with anti-Bcl-XL rabbit antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bcl-XL antibody

Human, Mouse, Rat

ELISA, IP, IF, IHC, WB

$146
Cat # M00181-4
20μL, 40μL, 100μL, 200μL

Anti-BCL-2 Rabbit Monoclonal Antibody

Human, Mouse, Rat

ELISA, WB

$146
Cat # M00040-5
20μL, 40μL, 100μL, 200μL

Anti-Bim Rabbit Monoclonal Antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bim antibody

Human, Mouse, Rat

ELISA, IP, IF, WB

$146
Cat # M01552-1
20μL, 40μL, 100μL, 200μL

Anti-BNIP-2 Antibody

Western Blot analysis of various cells using BNIP-2 Polyclonal Antibody

Western Blot analysis of K562 cells using BNIP-2 Polyclonal Antibody

Western blot analysis of lysate from Jurkat cells, using BNIP-2 antibody

Human, Mouse

ELISA, IF, IHC, WB

$399
Cat # A07336
100μl

Anti-BCL2L11 Antibody (Center)

Western blot analysis of BCL2L11 Antibody (Center) in K562, HL-60 cell line lysates (35ug/lane)

Immunohistochemical analysis of paraffin-embedded H

Human

Flow Cytometry, IHC-P, WB

$299
Cat # A01552-3
80 µl

Anti-MCL1 Antibody (BH3 Domain Specific)

Formalin-fixed and paraffin-embedded human breast carcinoma with MCL1 Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining

Human, Mouse, Rat

Flow Cytometry, IHC-P, WB

$299
Cat # A00712-1
80 µl

Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody

Western Blot analysis of HT-29 cells using CYP2R1 Polyclonal Antibody

Western blot analysis of lysates from HT29 cells, using Cytochrome P450 2R1 Antibody

Western blot analysis of the lysates from HT-29 cells using Cytochrome P450 2R1 antibody

Human, Monkey, Mouse

ELISA, IF, IHC, WB

$399
Cat # A03557
100μl

Bcl G (BCL2L14) (NM_138722) Human Over-expression Lysate

Bcl G (BCL2L14) Human Over-expression Lysates NM_030766

Human

$628
Cat # LS079370
20 µg, 100 µg

Anti-Phospho-Bcl-x (T47) BCL2L1 Antibody

Western blot analysis of RAT-MUSLE using Phospho-Bcl-x (T47) antibody

Western blot analysis of lysates from 293 cells treated with UV 30

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BCL-XL (Phospho-Thr47) Antibody

Human, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A00181T47-1
100ul

Anti-Phospho-Bcl-2 (S87) Antibody

Western Blot analysis of 293T-UV cells using Phospho-Bcl-2 (S87) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from HeLa cells treated with nocodazole 1ug/ml 18h, using BCL-2 (Phospho-Ser87) Antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using BCL-2 (Phospho-Ser87) Antibody

Human, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A00040S87
100ul

Anti-Phospho-Bad (S91) Antibody

Western blot analysis of lysates from COS7 cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5', using BAD (Phospho-Ser91/128) Antibody

Western blot analysis of lysates from COS7 cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BAD (Phospho-Ser91/128) Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BAD (Phospho-Ser91/128) Antibody

Human, Monkey, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A03520S91-1
100ul

Anti-Phospho-Bad (S155) Antibody

Western Blot analysis of various cells using Phospho-Bad (S155) Polyclonal Antibody

Western blot analysis of lysates from 293 cells treated with Forskolin, using BAD (Phospho-Ser155) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BAD (Phospho-Ser155) Antibody

Human, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A03520S155-1
100ul

Anti-Phospho-Bad (S112) Antibody

Western Blot analysis of various cells using Phospho-Bad (S112) Polyclonal Antibody

Western blot analysis of lysates from 293 cells treated with Forskolin, using BAD (Phospho-Ser112) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BAD (Phospho-Ser112) Antibody

Human, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A03520S112-1
100ul

Anti-ARPP-21 Antibody

Western Blot analysis of HeLa cells using ARPP-21 Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HeLa cells, using ARPP21 Antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using ARPP21 Antibody

Human, Mouse

ELISA, IF, IHC, WB

$399
Cat # A03525-1
100μl

BCL-2 (Phospho-Thr56) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from K562 cells treated with H2O2 100uM 30

Human

ELISA

$540
Cat # EKC2363
1 kit, containing two 96-well plates and all necessary reagents

BCL-2 (Phospho-Ser70) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells treated with LPS (40nM

Human

ELISA

$540
Cat # EKC2362
1 kit, containing two 96-well plates and all necessary reagents

Bcl2 Binding component 3 (BBC3) (NM_001127240) Human Over-expression Lysate

Bcl2 Binding component 3 (BBC3) Human Over-expression Lysates NM_001127240

Human

$628
Cat # LS027113
20 µg, 100 µg

BCL2 (NM_000633) Human Over-expression Lysate

BCL2 Human Over-expression Lysates NM_000633

Human

$628
Cat # LS000596
20 µg, 100 µg

Anti-Bcl-B BCL2L10 Antibody

Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 μg/mL

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 μg/mL

Human

ELISA, ICC, WB

$481
Cat # A07380
0.1 mg

Anti-BCLAF1 Antibody

Western blot analysis of BCLAF1 in human brain tissue lysate with BCLAF1 antibody at 1 μg/ml

Immunohistochemistry of BCLAF1 in mouse brain tissue with BCLAF1 antibody at 5 μg/mL

Human, Mouse, Rat

ELISA, IHC-P, WB

$481
Cat # A04100-1
0.1 mg

Anti-BNIP3 Antibody

ICC staining BNIP3 in Hela cells (green)

Human, Mouse, Rat

IF, IHC, ICC, WB

$405
Cat # A01469-2
100ul

BAD (Phospho-Ser112) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from 293 cells treated with Forskolin

Human, Mouse, Rat

ELISA

$540
Cat # EKC2358
1 kit, containing two 96-well plates and all necessary reagents

BAD (Phospho-Ser155) Colorimetric Cell-Based ELISA Kit

Human, Mouse, Rat

ELISA

$540
Cat # EKC2357
1 kit, containing two 96-well plates and all necessary reagents

BIM Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from COS7 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1061
1 kit, containing one 96-well plate and all necessary reagents

Anti-Rap1GAP Antibody

Western Blot (WB) analysis of COLO205 cells using Rap1GAP Polyclonal antibody

Western Blot (WB) analysis of specific cells using Rap1GAP Polyclonal antibody

Human, Mouse

ELISA, IF, IHC, WB

$399
Cat # A03561
100μl

Anti-Ribosomal Protein S2 RPS2 Antibody

Western Blot analysis of various cells using Ribosomal Protein S2 Polyclonal Antibody diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded human oophoroma

Human, Mouse, Rat

ELISA, IHC, WB

$399
Cat # A03548-1
100 μl

BCL2 Overview

Facts about Apoptosis regulator Bcl-2.

BCL2 3D structure. Source: alphafold

Apoptosis regulator Bcl-2 (BCL2)

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability.

Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria or by binding to the apoptosis-activating variable (APAF-1).

Gene Information

Human
Gene Name:	BCL2
Uniprot:	P10415
Entrez:	596

Family

Belongs to:

Bcl-2 family

Alternative Names

apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2

Molecular Weight

Mass (kDA):
26.266 kDA

Genomic Context


Human

Location:	18q21.33
Sequence:	18; NC_000018.10 (63123346..63320280, complement)

Tissue Specificity

Expressed in a variety of tissues.

Subcellular Localizations

Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Diseases

Neoplasms
Malignant Neoplasms
Lymphoma
B-cell Lymphomas
Carcinoma
Leukemia
Lymphoma, Follicular
Diffuse Large B-cell Lymphoma
Chromosomal Translocation
Malignant Paraganglionic Neoplasm
Malignant Neoplasm Of Breast
Mammary Neoplasms

Cell Transformation, Neoplastic
Lymphoma, Non-hodgkin
Neoplasm Metastasis
Chronic Lymphocytic Leukemia
Lymphoid Leukemia
Cytogenetic Abnormality
Myeloid Leukemia

Interacting Proteins

AMBRA1
BAD
Bad
BAK1
BAX

BBC3
BCAP31
BCL2L11
BCLAF1
BECN1

BID
BIK
Bmf
BNIP3L
BRCA1

CISD2
Itpr1
LRRK2
MDM4
NLRP1

NR4A1
PMAIP1
SIVA1
TP53
TP53BP2

Pathways

Cell Death
Cell Cycle
Cell Proliferation
Pathogenesis
Induction Of Apoptosis
Cell Growth
Programmed Cell Death
Cell Cycle Arrest
Localization
Interphase
Autophagy
Angiogenesis
Methylation

Aging
Cell Differentiation
Drug Resistance
Dna Repair
Translation
Secretion
Immune Response

PTMs

Phosphorylation
Cleavage
Methylation
Dephosphorylation
Reduction
Oxidation
Acetylation
Phosphorothioation
Ubiquitination

Demethylation
Deacetylation
Glycosylation
Carboxylation
Decarboxylation
Phosphorothionation
Citrullination
Acylation
Amination

Deglycosylation

Research Areas

Apoptosis
Autophagy
Cancer
Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BCL2

References

PMID: 3523487 by Tsujimoto Y., et al. Analysis of the structure, transcripts, and protein products of bcl- 2, the gene involved in human follicular lymphoma.

PMID: 1508712 by Eguchi Y., et al. Isolation and characterization of the chicken bcl-2 gene: expression in a variety of tissues including lymphoid and neuronal organs in adult and embryo.

324 Publications of Boster Apoptosis regulator Bcl-2 Antibodies & ELISA Kits

PubMed ID: PMID:28559975, Inhibition of glioblastoma growth and invasion by 125I brachytherapy in rat glio...
PubMed ID: PMID:28386340, Lipoxin A4 pretreatment mitigates skeletal muscle ischemia-reperfusion injury in...
PubMed ID: PMID:27122753, The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats
PubMed ID: PMID:26464676, Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer
PubMed ID: PMID:26191239, Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma a...
PubMed ID: PMID:26064215, Effects of PDTC on NF-κB expression and apoptosis in rats with severe acute pan...
PubMed ID: PMID:25785006, Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative co...
PubMed ID: 10.4314/tjpr.v15i2.7, A Study on Expression and Tyrosine 705 phosphorylation of STAT3 in Focal Cerebra...
PubMed ID: 10.4103/1673-5374.177736, Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone mar...
PubMed ID: 10.3892/or.2016.4625, The role of cofilin-l in vulvar squamous cell carcinoma: A marker of carcinogene...
PubMed ID: 10.3892/or.2015.3725, Cycloartan-24-ene-1α,2α,3β-triol, a cycloartane-type triterpenoid from the re...
PubMed ID: 10.3892/ol.2013.1103, Characterization of side population cells isolated from the gastric cancer cell ...
PubMed ID: 10.3892/mmr.2019.10208, Ghrelin regulates sepsis‑induced rat acute gastric injury
PubMed ID: 10.3892/etm.2014.1690, Effect of ligustilide on Ang II‑induced hypertrophy in cardiomyocytes and the...
PubMed ID: 10.3748/wjg.v11.i29.4579, Effects of garlicin on apoptosis in rat model of colitis
PubMed ID: 10.3724/SP.J.1009.2011.00450, Neuroprotective Effects of Hyperoside on Sodium Azide-Induced Apoptosis in PC12 ...
PubMed ID: 10.3390/toxins13070503, The Protein-Independent Role of Phosphate in the Progression of Chronic Kidney D...
PubMed ID: 10.3390/i7020059, Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by S...
PubMed ID: 10.3389/fphar.2017.00691, Protective Effects of Sodium (±)-5-Bromo-2-(α-Hydroxypentyl) Benzoate in a Rod...
PubMed ID: 10.3389/fphar.2017.00044, Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Au...
PubMed ID: 10.3389/fcell.2021.684857, Tunicamycin Induces Hepatic Stellate Cell Apoptosis Through Calpain-2/Ca2 +-Depe...

*Showing only the more recent 20. More publications can be found for each product on its corresponding product page