Anti-Bcl-2 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

Western blot analysis of Bcl-2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:300 dilution.

Immunohistochemical analysis of paraffin-embedded Human tonsil, using the Antibody at 1:300 dilution.

Immunohistochemical analysis of paraffin-embedded human tonsil, using Bcl-2 Antibody.

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Immunofluorescent analysis of Jurkat cells, using Bcl-2 Antibody .

a Representative photographs of Bcl-2 immunological histological chemistry examination (200 ×). I: Control group, II: Elemene 20 mg/kg group, III: Elemene 40 mg/kg group, IV: Elemene 60 mg/kg group. b Quantification of P53 stained cells. * P < 0.05, ** P < 0.01 vs control group
Index in PubMed under a CC BY license. PMID: 31215421

TMEM16A bound with p62 and modulated p62-Bcl-2-Beclin-1-VPS43 complex formation. (A and B) Immunoprecipitation (IP) followed by immunoblotting (IB) in MASMC lysates showing presence of p62 in TMEM16A immunoprecipitates (A), and reciprocal immunoprecipitation showing the presence of TMEM16A in p62 immunoprecipitates (B). (C and D) MASMCs were co-transfected with vectors expressing TMEM16A tagged with HA and RFP, and p62 tagged with His. Western blot analysis for His (C) or HA (D) after immunoprecipitation with an HA or His antibody, respectively. n=5. (E-H) The cells were infected with Ad-Lacz or Ad-TM adenovirus (E and F, respectively), or transfected with scr.RNA or TM siRNA (G and H, respectively) for 48 h and then treated with AngII for another 24 h. Lysates were immunoprecipitated with a Bcl-2 (E and G) or Beclin-1 (F and H) antibody and immunoblotted with the indicated antibodies. **P < 0.01 vs. control; ##P < 0.01 vs. AngII, one-way ANOVA. n = 7. (I) Schematic representation of the study findings. TMEM16A bound with p62 and sequestered it from the p62-Bcl-2 complex. AngII-induced TMEM16A downregulation and led to reduced TMEM16A-p62 association and facilitated the binding of p62-Bcl-2, which limited formation of Bcl-2-Beclin-1 complexes. Finally, the enhanced Beclin-1-VPS34 association increased the kinase activity of VPS34 and initiated autophagy, leading to VSMC hyperplasia and remodeling.
Index in PubMed under a CC BY license. PMID: 32226533

a Representative photographs of P53 and Bcl-2 expression on xenograft tumor lysate. b : Normalize grey values of P53 and Bcl-2 expression. * P < 0.05, ** P < 0.01 vs control group
Index in PubMed under a CC BY license. PMID: 31215421

Mice reconstituted with FGF2 KO macrophages and subjected to CLP exhibit increased proinflammatory gene expression and apoptosis. a - c The expression of the inflammatory factors CXCL1, IL1β, and IL6 in lung tissue was detected by real-time PCR ( n = 3 per group). d TUNEL staining was used to evaluate apoptosis in the lung tissue. e - f The apoptosis-associated genes BCL2 and Bax were identified by western blot analysis. Bar is 20 μm. * p < 0.05, vs. WT; Δ p < 0.05, vs. WT + LPS; # p < 0.05 vs. FGF2 KO
Index in PubMed under a CC BY license. PMID: 39436561

TGF-β1 suppresses the HCC cells proliferative capacity but does not promote apoptosis. SMMC-7721 and BEL-7402 cells were treated with 0, 5ng/mL and 10ng/mL TGF-β1 for 48 hours. A . apoptosis B . western blotting examined the expression levels of Bcl-2 and Bax. Data are expressed as the mean ± SEM of three independent experiments, #P>0.05.
Index in PubMed under a CC BY license. PMID: 28076850