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BCL2 antibody (M00040-2) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 23 Publications

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Anti-Bcl-2 Rabbit Monoclonal Antibody

Name: Anti-Bcl-2 Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: M00040-2
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

BCL2 antibody

23 Publications|

1 Review

|Submit a Review

Reactive Species	Host	Application
Human, Mouse	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

BCL2 antibody was positively detected by IHC in:

Human lung adenocarcinoma tissue, Human thyroid cancer tissue, Human tonsil tissue, human tonsil tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Recommend

Tested

Recommend

Mouse

Recommend

Product Information

Product Name

Anti-Bcl-2 Rabbit Monoclonal Antibody
View all BCL2 Antibodies

Form

Liquid

Uniprot

P10415

Gene ID

596

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

AAOH-2

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Bcl-2

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

26 kDa

Calculated Molecular Weight

26.3 kDa

Cite This Product

Anti-Bcl-2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00040-2)

Protocols

Immunohistochemistry (IHC) protocol for BCL2 antibody

Review Protocol

Publications

Cordycepin protects against simulated microgravity–induced osteogenic impairment involving ferroptosis-related signaling

Published: 2026-08-16

Journal: BIOCHEMICAL PHARMACOLOGY

Application: WB

Impact Factor: 6.50

Species: Mouse

PMID: 42603620

SKU	M00040-2
Application	Western Blot
Sample	mouse lung tissue
Sample Processing Description	Total protein was extracted from the lung tissues of three normal mice.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	Bcl-2 Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	BCL2 acts as a key regulator of cell survival and death decisions. It protects cells through multiple mechanisms, including preventing mitochondrial-mediated apoptosis, maintaining endoplasmic reticulum homeostasis, and regulating autophagy under nutrient-rich conditions. In this experiment, three normal lung tissue samples were used to validate the performance of the BCL2 antibody. The results showed a clear target band at the expected position, demonstrating good antibody performance.

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Anti-Bcl-2 Rabbit Monoclonal Antibody

BCL2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Cordycepin protects against simulated microgravity–induced osteogenic impairment involving ferroptosis-related signaling

Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion

Brown adipose tissue-derived FGF21 mediates the cardioprotection of dexmedetomidine in myocardial ischemia/reperfusion injury

Induction of apoptosis and autophagy by dichloromethane extract from Patrinia scabiosaefolia Fisch on acute myeloid leukemia cells

Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents

Neuroprotective Effects of a Cholecystokinin Analogue in the 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine Parkinson’s Disease Mouse Model

MiR-325-3p Alleviates Acute Pancreatitis via Targeting RIPK3

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis

Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate

Protective effects of safranal on hypoxia/reoxygenation‑induced injury in H9c2 cardiac myoblasts via the PI3K/AKT/GSK3β signaling pathway

Allicin suppresses growth and metastasis of gastric carcinoma: the key role of microRNA-383-5p-mediated inhibition of ERBB4 signaling

Crocin ameliorates arsenic trioxide‑induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis

TMEM16A ameliorates vascular remodeling by suppressing autophagy via inhibiting Bcl-2-p62 complex formation

Polysaccharide from Echinacea purpurea reduce the oxidant stress in vitro and in vivo

Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice

Anti-tumor effect and mechanistic study of elemene on pancreatic carcinoma

Biological characteristics of heat shock protein 90 in human liver cancer cells

Tumor necrosis factor-related apoptosis inducing ligand overexpression and Taxol treatment suppresses the growth of cervical cancer cells in vitro and in vivo

The antitumor effect of folic acid conjugated-Auricularia auricular polysaccharide-cisplatin complex on cervical carcinoma cells in nude mice

Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling

Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line

The inhibition of Endostar on the angiogenesis and growth of gastrointestinal stromal tumor xenograft

Effects of Chinese medicinal fungus water extract on tumor metastasis and some parameters of immune function

Review

Have you used Anti-Bcl-2 Rabbit Monoclonal Antibody?

1 Review For Anti-Bcl-2 Rabbit Monoclonal Antibody

The BCL2 antibody (M00040-2) was validated by Western blot using normal mouse lung tissues. The target band was clear and detected at the expected molecular weight, demonstrating excellent performance and reliable specificity.

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