Anti-BCL2A1/Bcl 2 Related Protein A1 Rabbit Monoclonal Antibody

Product Name

Anti-BCL2A1/Bcl 2 Related Protein A1 Rabbit Monoclonal Antibody

View all Bcl-2 related protein A1 Antibodies

SKU/Catalog Number

M03850

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-BCL2A1/Bcl 2 Related Protein A1 Rabbit Monoclonal Antibody catalog # M03850. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BCL2A1/Bcl 2 Related Protein A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03850)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EEE-2

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human BCL2A1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03850 is reactive to BCL2A1 in Human, Mouse, Rat

Applications

M03850 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

20.132kDa

Background of Bcl-2 related protein A1

Plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
FC 1:100

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BCL2A1 using anti-BCL2A1 antibody (M03850).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2A1 antigen affinity purified monoclonal antibody (Catalog # M03850) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2A1 at approximately 27 kDa. The expected band size for BCL2A1 is at 20 kDa.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Gene/Protein Information For BCL2A1 (Source: Uniprot.org, NCBI)

Alternative Names

ACC-1; ACC-2; Bcl-2 related protein A1; BCL2A1; Bcl2L5; Bcl2-L-5; BCL2L5HBPA1; Bcl-2-like protein 5; BCL2-related protein A1; Bfl-1; BFL1bcl-2-related protein A1; GRS; GRSbcl2-L-5; hematopoietic BCL2-related protein A1; Hemopoietic-specific early response protein; Protein BFL-1; Protein GRS BCL2A1 ACC-1, ACC-2, ACC1, ACC2, BCL2L5, BFL1, GRS, HBPA1 BCL2 related protein A1 bcl-2-related protein A1|bcl-2-like protein 5|bcl2-L-5|hematopoietic BCL2-related protein A1|hemopoietic-specific early response protein|protein BFL-1

For more info on BCL2A1, check out the BCL2A1 Infographic

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In this infographic, you will see the following information for BCL2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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