Anti-beta 2 Microglobulin/B2m Antibody Picoband®

Western blot analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody (A00456-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: rat small intestine tissue lysates,
Lane 6: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta 2 Microglobulin/B2m antigen affinity purified polyclonal antibody (Catalog # A00456-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta 2 Microglobulin/B2m at approximately 13KD. The expected band size for Beta 2 Microglobulin/B2m is at 13KD.

IF analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody (A00456-2).
Beta 2 Microglobulin/B2m was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Beta 2 Microglobulin/B2m Antibody (A00456-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody (A00456-2).
Beta 2 Microglobulin/B2m was detected in immunocytochemical section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Beta 2 Microglobulin/B2m Antibody (A00456-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Flow Cytometry analysis of RH35 cells using anti-Beta 2 Microglobulin/B2m antibody (A00456-2).
Overlay histogram showing RH35 cells stained with A00456-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Beta 2 Microglobulin/B2m Antibody (A00456-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.