Anti-Beta-2 Microglobulin (Renal Failure & Tumor Marker) B2M Monoclonal Antibody

Product Name

Anti-Beta-2 Microglobulin (Renal Failure & Tumor Marker) B2M Monoclonal Antibody

View all beta 2-Microglobulin Antibodies

SKU/Catalog Number

M00456

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Beta-2 Microglobulin (Renal Failure & Tumor Marker) B2M Monoclonal Antibody (Catalog # M00456). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Primate.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Beta-2 Microglobulin (Renal Failure & Tumor Marker) B2M Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00456)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: B2M/961

Isotype

IgG2b, kappa

Immunogen

Full length recombinant human B2M protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00456 is reactive to B2M in Human, Primate

Applications

M00456 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

13.715kDa

Background of beta 2-Microglobulin

Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with 1ml water (15 minutes at room temperature).

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-4ug/ml)
Western Blot (0.5-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human melanoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Western Blot analysis of THP-1 and Raji cell lysates using Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Immunofluorescence analysis of HeLa cells labeling with Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot (red).

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Flow Cytometric analysis of human HeLa cells. Anti-Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Gene/Protein Information For B2M (Source: Uniprot.org, NCBI)

Alternative Names

B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin B2M IMD43 beta-2-microglobulin beta-2-microglobulin|beta chain of MHC class I molecules|beta-2-microglobin

For more info on B2M, check out the B2M Infographic

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In this infographic, you will see the following information for B2M: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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