Anti-BIN1 Monoclonal Antibody

Product Name

Anti-BIN1 Monoclonal Antibody

View all BIN1 Antibodies

SKU/Catalog Number

M01551-2

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-BIN1 Monoclonal Antibody (Catalog # M01551-2). Tested in IF, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-BIN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01551-2)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 99F

Isotype

IgG

Immunogen

Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with BIN1 polypeptide followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~85kDa.

Reactive Species

M01551-2 is reactive to BIN1 in Human, Mouse

Applications

M01551-2 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

64.699kDa

Background of BIN1

Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells and play a role in cancer suppression. Studies in muscle cells suggest that Bin1 expression, structure, and localization are tightly regulated during muscle differentiation and suggested that Bin1 plays a functional role in the differentiation process. Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5000 - 1:50000
IF Microscopy: 1:100-1:500
IP: 10-100 µL
WB: 1:500-1:1500

Validation Images & Assay Conditions

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Immunoprecipitation of Mouse anti-BINBin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody.Lane 1: BIN 1Lane 2:none.Load:35

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Immunofluorescence Microscopy of Mouse Anti-BIN1 Antibody.Cells: C2C12 cells during growth or differentiation.Fixation:0.5% PFA.Antigen retrieval:not required.Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody.Secondary antibody:mouse secondary antibody at 1:10,000 for 45 min at RT.Localization:BIN1 is nuclear and cytoplasmic.Staining:BIN 1 as green fluorescent signal.

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Figure 3. Western blot analysis of BIN1 using anti-BIN1 antibody (M01551-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIN1 antigen affinity purified polyclonal antibody (Catalog # M01551-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for BIN1.

Gene/Protein Information For BIN1 (Source: Uniprot.org, NCBI)

Alternative Names

AMPH2; Amphiphysin II; Amphiphysin-like protein; AMPHLDKFZp547F068; box dependant MYC interacting protein 1; bridging integrator 1Box-dependent myc-interacting protein 1; MGC10367; myc box-dependent-interacting protein 1; SH3P9 BIN1 AMPH2, AMPHL, CNM2, SH3P9 bridging integrator 1 myc box-dependent-interacting protein 1|amphiphysin II|amphiphysin-like protein|box dependant MYC interacting protein 1|box-dependent myc-interacting protein 1

For more info on BIN1, check out the BIN1 Infographic

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In this infographic, you will see the following information for BIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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