Product Info Summary
SKU: | A00260-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-BMAL1/ARNTL Antibody Picoband™
SKU/Catalog Number
A00260-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BMAL1/ARNTL Antibody Picoband™ catalog # A00260-1. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMAL1/ARNTL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00260-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BMAL1/ARNTL recombinant protein (Position: H58-D156). Human BMAL1/ARNTL shares 99% and 98% amino acid (aa) sequence identity with mouse and rat BMAL1/ARNTL, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00260-1 is reactive to ARNTL in Human
Applications
A00260-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
69 kDa
Calculated molecular weight
68.762kDa
Background of BMAL1
Aryl hydrocarbon receptor nuclear translocator-like protein 1 is protein that in humans is encoded by the ARNTL gene. The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates,
Lane 2: HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 69KD. The expected band size for BMAL1/ARNTL is at 69KD.
Protein Target Info & Infographic
Gene/Protein Information For ARNTL (Source: Uniprot.org, NCBI)
Gene Name
ARNTL
Full Name
Aryl hydrocarbon receptor nuclear translocator-like protein 1
Weight
68.762kDa
Alternative Names
aryl hydrocarbon receptor nuclear translocator-like protein 1; aryl hydrocarbon receptor nuclear translocator-like; basic-helix-loop-helix-PAS orphan MOP3; BHLHE5; bHLHe5brain and muscle; bHLH-PAS protein JAP3; BMAL1TIC; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5; Member of PAS protein 3; member of PAS superfamily 3; MOP3BMAL1c; PAS domain-containing protein 3; PASD3MGC47515 ARNTL BMAL1, BMAL1c, JAP3, MOP3, PASD3, TIC, bHLHe5 aryl hydrocarbon receptor nuclear translocator like aryl hydrocarbon receptor nuclear translocator-like protein 1|ARNT-like protein 1, brain and muscle|PAS domain containing 3|PAS domain-containing protein 3|bHLH-PAS protein JAP3|basic helix-loop-helix family member e5|basic-helix-loop-helix-PAS orphan MOP3|basic-helix-loop-helix-PAS protein MOP3|brain and muscle ARNT-like 1|class E basic helix-loop-helix protein 5|member of PAS protein 3|member of PAS superfamily 3|testis tissue sperm-binding protein Li 50e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARNTL, check out the ARNTL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARNTL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BMAL1/ARNTL Antibody Picoband™ (A00260-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-BMAL1/ARNTL Antibody Picoband™
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-BMAL1/ARNTL antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
According to literature brain does express ARNTL. According to Uniprot.org, ARNTL is expressed in left lobe of thyroid gland, brain, fetal brain, brain skin, among other tissues. Regarding which tissues have ARNTL expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9144434, 14702039
Brain, and Skin, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 9079689
Boster Scientific Support
Answered: 2019-09-19
Question
Has A00260-1 Antibody been tested to react on mouse or rat BMAL1?
Verified customer
Asked: 2019-07-17
Answer
The Anti-BMAL1/ARNTL Antibody Picoband A00260-1 didn't work well on mouse and rat samples.
Boster Scientific Support
Answered: 2019-07-18
Question
We need using your anti-BMAL1/ARNTL antibody for maternal process involved in parturition studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We appreciate your inquiry. This A00260-1 anti-BMAL1/ARNTL antibody is tested on hela whole cell lysates, hepg2 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-22
Question
We were happy with the WB result of your anti-BMAL1/ARNTL antibody. However we have seen positive staining in fetal brain nucleus using this antibody. Is that expected? Could you tell me where is ARNTL supposed to be expressed?
G. Krishna
Verified customer
Asked: 2018-06-01
Answer
Based on literature, fetal brain does express ARNTL. Generally ARNTL expresses in nucleus. Regarding which tissues have ARNTL expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9144434, 14702039
Brain, and Skin, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 9079689
Boster Scientific Support
Answered: 2018-06-01
Question
We are currently using anti-BMAL1/ARNTL antibody A00260-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
G. Banerjee
Verified customer
Asked: 2017-11-08
Answer
The anti-BMAL1/ARNTL antibody (A00260-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-08