Anti-BMPR2 Antibody Picoband™

Product Name

Anti-BMPR2 Antibody Picoband™

View all BMPR-II Antibodies

SKU/Catalog Number

A00324-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BMPR2 Antibody Picoband™ catalog # A00324-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BMPR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00324-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human BMPR2 recombinant protein (Position: R455-K512).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00324-1 is reactive to BMPR2 in Human, Mouse, Rat

Applications

A00324-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

130 kDa

Calculated molecular weight

115.201kDa

Background of BMPR-II

Bone morphogenetic protein receptor type II or BMPR2 is a serine/threonine receptor kinase. This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of BMPR2 using anti-BMPR2 antibody (A00324-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR2 antigen affinity purified polyclonal antibody (Catalog # A00324-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR2 at approximately 130KD. The expected band size for BMPR2 is at 115KD.

Gene/Protein Information For BMPR2 (Source: Uniprot.org, NCBI)

Alternative Names

BMP type II receptor; BMP type-2 receptor; BMPR2; BMPR-2; BMPR3; BMPRII; BMPR-II; BMPR-IIprimary pulmonary hypertension 1; Bone morphogenetic protein receptor type II; bone morphogenetic protein receptor type-2; bone morphogenetic protein receptor, type II (serine/threonine kinase); BRK-3; EC 2.7.11; EC 2.7.11.30; FLJ41585; FLJ76945; PPH1BMR2; T-ALK; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4 BMPR2 BMPR-II, BMPR3, BMR2, BRK-3, POVD1, PPH1, T-ALK bone morphogenetic protein receptor type 2 bone morphogenetic protein receptor type-2|BMP type II receptor|BMP type-2 receptor|bone morphogenetic protein receptor type II|bone morphogenetic protein receptor, type II (serine/threonine kinase)|type II activin receptor-like kinase|type II receptor for bone morphogenetic protein-4

For more info on BMPR2, check out the BMPR2 Infographic

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In this infographic, you will see the following information for BMPR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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