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Product Info Summary
| SKU: | A04690 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BRCC36 Antibody
SKU/Catalog Number
A04690
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BRCC36 Antibody (Catalog # A04690). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BRCC36 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BRCC36 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04690)
Host
Rabbit
Contents
BRCC36 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. The immunogen is located within amino acids 20 - 70 of BRCC36.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.
Reactive Species
A04690 is reactive to BRCC3 in Human, Mouse, Rat
Applications
A04690 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
36.072kDa
Background of BRCC3
Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of BRCC36 in human brain tissue with BRCC36 antibody at 2.5 μg/mL.
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Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BRCC3 (Source: Uniprot.org, NCBI)
Gene Name
BRCC3
Full Name
Lys-63-specific deubiquitinase BRCC36
Weight
36.072kDa
Superfamily
peptidase M67A family
Alternative Names
BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRCA1/BRCA2-containing complex, subunit 3; BRCA1-A complex subunit BRCC36; BRCC36C6.1ACXorf53; BRISC complex subunit BRCC36; c6.1A; chromosome X open reading frame 53; EC 3.1.2.15; EC 3.4.19.-; lys-63-specific deubiquitinase BRCC36 BRCC3 BRCC36, C6.1A, CXorf53 BRCA1/BRCA2-containing complex subunit 3 lys-63-specific deubiquitinase BRCC36|BRCA1-A complex subunit BRCC36|BRCA1/BRCA2-containing complex subunit 36|BRISC complex subunit BRCC36
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRCC3, check out the BRCC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRCC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRCC36 Antibody (A04690)
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Customer Reviews
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