Anti-BRI3B BRI3BP Antibody

Bri3bp antibody

Boster Bio Anti-BRI3B BRI3BP Antibody (Catalog # A13573). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A13573
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

Product Name

Anti-BRI3B BRI3BP Antibody

View all Bri3bp Antibodies

SKU/Catalog Number

A13573

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-BRI3B BRI3BP Antibody (Catalog # A13573). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BRI3B BRI3BP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13573)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human BRI3B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A13573 is reactive to Bri3bp in Human, Mouse

Applications

A13573 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

28.263kDa

Background of Bri3bp

Involved in tumorigenesis and may function by stabilizing p53/TP53.

Lin L., Biochem. Genet. 39:369-377(2001).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Kim J.W., Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For Bri3bp (Source: Uniprot.org, NCBI)

Gene Name

Bri3bp

Full Name

BRI3-binding protein

Weight

28.263kDa

Alternative Names

BNAS1; BRI3 binding protein; BRI3-binding protein; Cervical cancer 1 proto-oncogene-binding protein KG19; cervical cancer oncogene binding protein; HCCR-1; HCCR-2; HCCRBP-1; KG19I3-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Bri3bp, check out the Bri3bp Infographic

Bri3bp infographic

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In this infographic, you will see the following information for Bri3bp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-BRI3B BRI3BP Antibody

Question

I see that the anti-BRI3B antibody A13573 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-23

Question

Is a blocking peptide available for product anti-BRI3B antibody (A13573)?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

We do provide the blocking peptide for product anti-BRI3B antibody (A13573). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-13

Question

Does A13573 anti-BRI3B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

It shows on the product datasheet, A13573 anti-BRI3B antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-30

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin {eco:0000312 using anti-BRI3B antibody A13573. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-27

Question

See attached the WB image, lot number and protocol we used for skin {eco:0000312 using anti-BRI3B antibody A13573. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-06

Question

My question regarding product A13573, anti-BRI3B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Patel

Verified customer

Asked: 2017-01-10

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13573 anti-BRI3B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-01-10

Order DetailsPrice
A13573-100uL

100uL

$360

Free Secondary Antibody

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A13573
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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