Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ANGPT2 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ0657
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all Angiopoietin-2 products

SKU/Catalog Number

EZ0657

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ANGPT2 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0657)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y19-F496

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0657 is reactive to ANGPT2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk.

Application Guarantee

EZ0657 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0657-CA Rabbit anti- human ANGPT2 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0657-DA Biotinylated goat anti- human ANGPT2 polyclonal antibody (Detection Antibody) 500 µl, 7 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0657-ST Lyophilized recombinant human ANGPT2 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0950.1780.210.3760.5190.971.5882.01

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3271634590034117695438
Standard deviation17.33114.3835423.52127.36342.59
CV (%)5.3%7%6%6.9%7.2%6.3%

Gene/Protein Information For ANGPT2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15123

Gene ID

285

Gene Name

ANGPT2

Full Name

Angiopoietin-2

Weight

56.919kDa

Alternative Names

AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand ANGPT2 AGPT2, ANG2 angiopoietin 2 angiopoietin-2|Tie2-ligand|angiopoietin-2B|angiopoietin-2a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANGPT2, check out the ANGPT2 Infographic

ANGPT2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANGPT2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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2 Customer Q&As for Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

Can EZ0657 kit be used with exosomes?

Verified customer

Asked: 2019-07-23

Answer

Our lab technicians haven't worked on exosomes with the Human Angiopoietin-2 ELISA Kit EZ-Set (DIY Antibody Pairs) EZ0657, so we cannot guarantee that it would work with the kit. It is suggested to run first a pilot test for this.

Boster Scientific Support

Answered: 2019-07-24

Question

Is it possible to use EZ0657 with exosomes? Are there any adjustments that you would recommend to make this work?

Verified Customer

Verified customer

Asked: 2017-10-26

Answer

For EZ0657, our lab technicians have not worked on exosomes, so we cannot guarantee that the kit will work with the samples nor can we provide any suggestions. We suggest running a pilot test.

Boster Scientific Support

Answered: 2017-10-26

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