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Product Info Summary
| SKU: | FEK1574 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Rat Angiopoietin-2 PicoKine® Quick ELISA Kit
See all Angiopoietin-2 products
SKU/Catalog Number
FEK1574
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Angiopoietin-2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Angpt2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Angiopoietin-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1574)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Y19-F496
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1574 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1574 is reactive to ANGPT2 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1574 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angiopoietin-2
Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1574-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1574-ST | Rat Angiopoietin-2 Standard | 2 | 10 ng/tube |
| FEK1574-A | Rat ANGPT2 Antibody A | 1 | 3ml |
| FEK1574-B | Rat ANGPT2 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.030 | 0.153 | 0.321 | 0.669 | 1.026 | 1.628 | 2.072 | 2.434 |
Data Example Images
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Rat Angiopoietin-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 265 | 1942 | 5069 | 245 | 1831 | 4768 |
| Standard deviation | 19.88 | 95.16 | 238.24 | 22.54 | 117.18 | 243.17 |
| CV (%) | 7.5% | 4.9% | 4.7% | 9.2% | 6.4% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 265 | 255 | 217 | 223 | 240 | 17.52 | 7.3% |
| Sample 2 | 1942 | 2098 | 1841 | 1995 | 1969 | 100.42 | 5.1% |
| Sample 3 | 5069 | 4532 | 4756 | 5147 | 4876 | 238.92 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For ANGPT2 (Source: Uniprot.Org, NCBI)
Gene Name
ANGPT2
Full Name
Angiopoietin-2
Weight
56.919kDa
Alternative Names
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand ANGPT2 AGPT2, ANG2 angiopoietin 2 angiopoietin-2|Tie2-ligand|angiopoietin-2B|angiopoietin-2a
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANGPT2, check out the ANGPT2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANGPT2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Angiopoietin-2 PicoKine® Quick ELISA Kit (FEK1574)
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