Anti-BST2/Tetherin Antibody Picoband™

Product Name

Anti-BST2/Tetherin Antibody Picoband™

View all BST2 Antibodies

SKU/Catalog Number

A00934-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BST2/Tetherin Antibody Picoband™ catalog # A00934-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-BST2/Tetherin Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00934-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human BST2/Tetherin recombinant protein (Position: N49-S161).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00934-3 is reactive to BST2 in Human

Applications

A00934-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

25-45 kDa

Calculated molecular weight

19.769kDa

Background of BST2

Tetherin, also known as bone marrow stromal antigen 2, is a lipid raft associated protein that in humans is encoded by the BST2 gene. Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BST2/Tetherin using anti-BST2/Tetherin antibody (A00934-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BST2/Tetherin antigen affinity purified polyclonal antibody (Catalog # A00934-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BST2/Tetherin at approximately 25-45 kDa. The expected band size for BST2/Tetherin is at 20 kDa.

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Figure 2. Flow Cytometry analysis of Raji cells using anti-BST2/Tetherin antibody (A00934-3).
Overlay histogram showing Raji cells stained with A00934-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BST2/Tetherin Antibody (A00934-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For BST2 (Source: Uniprot.org, NCBI)

Alternative Names

bone marrow stromal antigen 2; bone marrow stromal cell antigen 2; BST2; BST-2; CD317 antigen; CD317; HM1.24 Antigen; NPC-A-7; PDCA-1; Tetherin BST2 CD317, HM1.24, TETHERIN bone marrow stromal cell antigen 2 bone marrow stromal antigen 2|BST-2|HM1.24 antigen|NPC-A-7|antiviral factor tetherin

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In this infographic, you will see the following information for BST2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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