Product Info Summary
SKU: | A01100-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-C/EBP Beta CEBPB Antibody
SKU/Catalog Number
A01100-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-C/EBP Beta CEBPB Antibody catalog # A01100-1. Tested in WB,ICC/IF,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C/EBP Beta CEBPB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01100-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human C4A, which shares 86.8% and 87.2% amino acid (aa) sequence identity with mouse and rat C4A, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01100-1 is reactive to CEBPB in Human, Mouse, Rat
Applications
A01100-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.106kDa
Background of CEBP Beta
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.
Gluck SL, et al. (2004) J Clin Invest; 114(12): 1696-1699
Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9784-9789
Tian D, et al. (2002) Mol Cell Biol; 22(8): 2650-2662
Lu Z, et al. (2001) Mol Cell Biol; 21(12): 4016-4031
Krishnan HH, et al. (2006) J Virol; 80(3): 1167-1180
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of CEBPB using Anti-C/EBP Beta CEBPB Antibody (A01100-1).
ICC staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-C/EBP Beta CEBPB Antibody A01100-1
Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For CEBPB (Source: Uniprot.org, NCBI)
Gene Name
CEBPB
Full Name
CCAAT/enhancer-binding protein beta
Weight
36.106kDa
Superfamily
bZIP family
Alternative Names
C/EBP beta; C/EBP-beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; CRP2; IL6DBP; interleukin 6-dependent DNA-binding protein; LAPTCF-5; Liver activator protein; liver-enriched transcriptional activator protein; MGC32080; NF-IL6; Nuclear factor NF-IL6; nuclear factor of interleukin 6; TCF5NFIL6; Transcription factor 5 CEBPB C/EBP-beta, IL6DBP, NF-IL6, TCF5 CCAAT enhancer binding protein beta CCAAT/enhancer-binding protein beta|CCAAT/enhancer binding protein (C/EBP), beta|interleukin 6-dependent DNA-binding protein|nuclear factor NF-IL6|nuclear factor of interleukin 6|transcription factor 5|transcription factor C/EBP beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEBPB, check out the CEBPB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEBPB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C/EBP Beta CEBPB Antibody (A01100-1)
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No publications found for A01100-1
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