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Product Info Summary
| SKU: | PA1555 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-c-Myc Antibody
SKU/Catalog Number
PA1555
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-c-Myc Antibody catalog # PA1555. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Myc Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1555)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human c-Myc, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1555 is reactive to MYC in Human, Mouse, Rat
Applications
PA1555 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
49 kDa
Calculated molecular weight
48.804kDa
Background of c-Myc
C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-c-Myc antibody, PA1555, Western blotting
All lanes: Anti c-Myc(PA1555) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
Protein Target Info & Infographic
Gene/Protein Information For MYC (Source: Uniprot.org, NCBI)
Gene Name
MYC
Full Name
Myc proto-oncogene protein
Weight
48.804kDa
Alternative Names
9E10 Myc; 9E10 Myc; avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian) MYC MRTLC, bHLHe39, c-Myc, MYC MYC proto-oncogene, bHLH transcription factor myc proto-oncogene protein|avian myelocytomatosis viral oncogene homolog|class E basic helix-loop-helix protein 39|myc-related translation/localization regulatory factor|proto-oncogene c-Myc|transcription factor p64|v-myc avian myelocytomatosis viral oncogene homolog|v-myc myelocytomatosis viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYC, check out the MYC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Myc Antibody (PA1555)
Hello CJ!
PA1555 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Long non-coding RNA LINC01503 predicts worse prognosis in glioma and promotes tumorigenesis and progression through activation of Wnt/β-catenin signaling H Wang 1, Z-G Sheng, L-Z Dai Eur Rev Med Pharmacol Sci. 2019 Feb;23(4):1600-1609. doi: 10.26355/e
Constitutive %u03B2-catenin activation in osteoblasts impairs terminal osteoblast differentiation and bone quality
Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma
Arginine deiminase expressed in vivo, driven by human telomerase reverse transcriptase promoter, displays high hepatoma targeting and oncolytic efficiency
Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression
Metformin Inhibits Tumorigenesis and Tumor Growth of Breast Cancer Cells by Upregulating miR-200c but Downregulating AKT2 Expression
Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2
Zhang D, Fei F, Li S, Zhao Y, Yang Z, Qu J, Zhang X, Yin Y, Zhang S. J Cancer. 2017 Jul 5;8(11):2114-2123. doi: 10.7150/jca.19723. eCollection 2017. The role of β-catenin in the initiation and metastasis of TA2 mice spontaneous breast cancer
Wang Ph, Gu Zh, Wan Dh, Zhu Wb, Qiu W, Weng Sp, Yu Xq, He Jg. Plos One. 2013;8(2):E52088. Doi: 10.1371/Journal.Pone.0052088. Epub 2013 Feb 6. Litopenaeus Vannamei Sterile-Alpha And Armadillo Motif Containing Protein (Lvsarm) Is Involved In Regulat...
Long Xb, Sun Gb, Hu S, Liang Gt, Wang N, Zhang Xh, Cao Pp, Zhen Ht, Cui Yh, Liu Z. Oncol Rep. 2009 Nov;22(5):1189-95. Let-7A Microrna Functions As A Potential Tumor Suppressor In Human Laryngeal Cancer.
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-c-Myc Antibody
Question
We are currently using anti-c-Myc antibody PA1555 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-c-Myc antibody (PA1555) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
My boss were satisfied with the WB result of your anti-c-Myc antibody. However we have been able to see positive staining in thoracic mammary gland nucleoplasm using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
Based on literature, thoracic mammary gland does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-01-04
Question
We have seen staining in rat cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-c-Myc antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
From literature cervix carcinoma erythroleukemia does express MYC. From Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-10-06
Question
My lab would like using your anti-c-Myc antibody for transcription of e2f targets under negative control by dream complex studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
K. Wu
Verified customer
Asked: 2016-09-30
Answer
Thanks for your inquiry. This PA1555 anti-c-Myc antibody is tested on hela whole cell lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-09-30
