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Product Info Summary
| SKU: | M00048 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-1 CASP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00048
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Caspase-1 CASP1 Rabbit Monoclonal Antibody catalog # M00048. Tested in WB, IHC applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-1 CASP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00048)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DCB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caspase-1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00048 is reactive to CASP1 in Human, Rat
Applications
M00048 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
83, 88 kDa
Calculated molecular weight
45.159kDa
Background of Caspase-1
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of Caspase-1 in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CASP1 (Source: Uniprot.org, NCBI)
Gene Name
CASP1
Full Name
Caspase-1
Weight
45.159kDa
Superfamily
peptidase C14A family
Alternative Names
CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta; Caspase1; Caspase-1; EC 3.4.22; EC 3.4.22.36; ICE; ICEP45; IL-1 beta-converting enzyme; IL1BC CASP1 nirs variant 1; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1-B converting enzyme; interleukin 1-beta convertase; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45 CASP1 ICE, IL1BC, P45 caspase 1 caspase-1|CASP1 nirs variant 1|IL-1 beta-converting enzyme|IL1B-convertase|caspase 1, apoptosis-related cysteine peptidase|caspase-1 isoform alpha|interleukin 1, beta, convertase|interleukin 1-B converting enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP1, check out the CASP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-1 CASP1 Rabbit Monoclonal Antibody (M00048)
Hello CJ!
M00048 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang XL,Huang WM,Tang PC,Sun Y,Zhang X,Qiu L,Yu BC,Zhang XY,Hong YX,He Y,Ge XQ.Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission.Neurotoxicology.2021 F
Species: Rat
M00048 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:NA
Yang R,Li Y,Mehmood S,Yan C,Huang Y,Cai J,Ji J,Pan W,Zhang W,Chen Y.Polysaccharides from Armillariella tabescens mycelia ameliorate renal damage in type 2 diabetic mice.Int J Biol Macromol.2020 Nov 1;162: 1682-1691.doi:10.1016/j.ijbiomac.2020.08.006.Epub
Species: Mouse
M00048 usage in article: APP:WB, SAMPLE:RENAL AND COLONIC TISSUE, DILUTION:NA
Fan X,Ma W,Zhang Y,Zhang L.P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury. Med Sci Monit.2020 Sep 21;26:e925491.doi:10.12659/MS
Species: Rat
M00048 usage in article: APP:WB, SAMPLE:SPINAL CORD, DILUTION:NA
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Caspase-1 CASP1 Rabbit Monoclonal Antibody
Question
My boss were content with the WB result of your anti-Caspase-1 Rabbit Monoclonal antibody. However we have been able to see positive staining in macrophage cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
According to literature, macrophage does express CASP1. Generally CASP1 expresses in cytoplasm. Regarding which tissues have CASP1 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 20148899
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-05-01
Question
We have tried in the past anti-Caspase-1 Rabbit Monoclonal antibody for IHC on leukocyte in the past. I am using rat, and We intend to use the antibody for WB next. I am interested in examining leukocyte as well as macrophage in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
I have checked the website and datasheets of our anti-Caspase-1 Rabbit Monoclonal antibody and it seems that M00048 has been validated on rat in both IHC and WB. Thus M00048 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-23
Question
I was wanting to use using your anti-Caspase-1 Rabbit Monoclonal antibody for interleukin-1 processing studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We appreciate your inquiry. This M00048 anti-Caspase-1 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-06
Question
I see that the anti-Caspase-1 Rabbit Monoclonal antibody M00048 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-13
Question
Here is the WB image, lot number and protocol we used for testis using anti-Caspase-1 Rabbit Monoclonal antibody M00048. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
We want to test anti-Caspase-1 Rabbit Monoclonal antibody M00048 on human testis for research purposes, then I may be interested in using anti-Caspase-1 Rabbit Monoclonal antibody M00048 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Anderson
Verified customer
Asked: 2019-10-22
Answer
The products we sell, including anti-Caspase-1 Rabbit Monoclonal antibody M00048, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-22
Question
Do you have a BSA free version of anti-Caspase-1 Rabbit Monoclonal antibody M00048 available?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-1 Rabbit Monoclonal antibody M00048 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-15
Question
Is a blocking peptide available for product anti-Caspase-1 Rabbit Monoclonal antibody (M00048)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
We do provide the blocking peptide for product anti-Caspase-1 Rabbit Monoclonal antibody (M00048). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-13
Question
Has M00048 been tested for IHC-F on human samples?
Verified customer
Asked: 2019-03-29
Answer
Unfortunately, we have not tested the Anti-Caspase-1 CASP1 Rabbit Monoclonal Antibody (M00048) for IHC-F on human samples. So, we do not know if they will work for IHC-F.
Boster Scientific Support
Answered: 2019-04-01
Question
My question regarding product M00048, anti-Caspase-1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00048 anti-Caspase-1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-08
Question
We are currently using anti-Caspase-1 Rabbit Monoclonal antibody M00048 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
The anti-Caspase-1 Rabbit Monoclonal antibody (M00048) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Caspase-1 Rabbit Monoclonal antibody M00048. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-15
Question
Will anti-Caspase-1 Rabbit Monoclonal antibody M00048 work for IHC with testis?
Verified Customer
Verified customer
Asked: 2017-11-29
Answer
According to the expression profile of testis, CASP1 is highly expressed in testis. So, it is likely that anti-Caspase-1 Rabbit Monoclonal antibody M00048 will work for IHC with testis.
Boster Scientific Support
Answered: 2017-11-29
Question
We have seen staining in human macrophage. Are there any suggestions? Is anti-Caspase-1 Rabbit Monoclonal antibody supposed to stain macrophage positively?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
According to literature macrophage does express CASP1. According to Uniprot.org, CASP1 is expressed in leukocyte, testis, macrophage, among other tissues. Regarding which tissues have CASP1 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 20148899
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-07-10
Question
Is this M00048 anti-Caspase-1 Rabbit Monoclonal antibody reactive to the isotypes of CASP1?
G. Baker
Verified customer
Asked: 2015-05-29
Answer
The immunogen of M00048 anti-Caspase-1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Caspase-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-05-29
Question
I was wanting to use your anti-Caspase-1 Rabbit Monoclonal antibody for IHC for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
E. Krishna
Verified customer
Asked: 2014-08-11
Answer
You can see on the product datasheet, M00048 anti-Caspase-1 Rabbit Monoclonal antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-11
Question
Will M00048 anti-Caspase-1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Taylor
Verified customer
Asked: 2013-07-01
Answer
As indicated on the product datasheet, M00048 anti-Caspase-1 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-07-01
