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Product Info Summary
| SKU: | A00334 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P |
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Product info
Product Name
Anti-Caspase-3 CASP3 Antibody
SKU/Catalog Number
A00334
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Caspase-3 CASP3 Antibody (Catalog # A00334). Tested in ELISA, IHC-P applications. This antibody reacts with Human.
Storage & Handling
Caspase-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Caspase-3 CASP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00334)
Host
Rabbit
Contents
Caspase-3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Caspase-3 antibody was raised against a 17 amino acid synthetic peptide near the center of human Caspase-3. The immunogen is located within amino acids 70 - 120 of Caspase-3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of Caspase-3 are known to exist.
Reactive Species
A00334 is reactive to CASP3 in Human
Applications
A00334 is guaranteed for ELISA, IHC-P Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.608kDa
Background of Caspase-3
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Caspase-3 antibody can be used for detection of Caspase-3 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Immunohistochemistry of Caspase-3 in human tonsil tissue with Caspase-3 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CASP3 (Source: Uniprot.org, NCBI)
Gene Name
CASP3
Full Name
Caspase-3
Weight
31.608kDa
Superfamily
peptidase C14A family
Alternative Names
Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP3, check out the CASP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-3 CASP3 Antibody (A00334)
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No publications found for A00334
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