Anti-Human Caspase-3 DyLight® 550 conjugated CASP3 Antibody

Caspase-3 antibody

Boster Bio Anti-Human Caspase-3 DyLight® 550 conjugated CASP3 Antibody catalog # A00334-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: A00334-Dyl550
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

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Product Name

Anti-Human Caspase-3 DyLight® 550 conjugated CASP3 Antibody

View all Caspase-3 Antibodies

SKU/Catalog Number

A00334-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human Caspase-3 DyLight® 550 conjugated CASP3 Antibody catalog # A00334-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human Caspase-3 DyLight® 550 conjugated CASP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00334-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Caspase-3 recombinant protein (Position: T67-D175). Human Caspase-3 shares 86% and 90% amino acid (aa) sequence identity with mouse and rat Caspase-3, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00334-Dyl550 is reactive to CASP3 in Human

Applications

A00334-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Background of Caspase-3

Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For CASP3 (Source: Uniprot.org, NCBI)

Uniprot ID

P42574

Gene ID

836

Gene Name

CASP3

Full Name

Caspase-3

Weight

31.608kDa

Superfamily

peptidase C14A family

Alternative Names

Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CASP3, check out the CASP3 Infographic

CASP3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CASP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

A00334-Dyl550 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Maternal separation exacerbates Alzheimer's disease-like behavioral and pathological changes in adult APPswe/PS1dE9 mice

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/%u03B2-catenin signaling

In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

Enhanced anti-apoptosis and gut epithelium protection function of acidic fibroblast growth factor after cancelling of its mitogenic activity

Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction

An J, Liu T, She R, Wu Q, Tian J, Shi R, Hao W, Ren X, Yang Y, Lu Y, Yang Y, Wu Y. Oncotarget. 2017 Dec 17;9(4):4475-4484. doi: 10.18632/oncotarget.23381. eCollection 2018 Jan 12. Replication of hepatitis E virus in the ovary and promotion of oocy...

Liu Nb, Peng T, Pan C, Yao Yy, Shen B, Leng J. World J Gastroenterol. 2005 Oct 28;11(40):6281-7. Overexpression Of Cyclooxygenase-2 In Human Hepg2, Bel-7402 And Smmc-7721 Hepatoma Cell Lines And Mechanism Of Cyclooxygenase-2 Selective Inhibitor Ce...

Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.

Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.

Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...

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14 Customer Q&As for Anti-Human Caspase-3 DyLight® 550 conjugated CASP3 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550. Let me know if you need anything else.

A. Rodriguez

Verified customer

Asked: 2020-05-08

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-05-08

Question

See below the WB image, lot number and protocol we used for t-cell using anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-14

Question

Is this A00334-Dyl550 anti-Human Caspase-3 DyLight® 550 conjugated antibody reactive to the isotypes of CASP3?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

The immunogen of A00334-Dyl550 anti-Human Caspase-3 DyLight® 550 conjugated antibody is E.coli-derived human Caspase-3 recombinant protein (Position: T67-D175). Human Caspase-3 shares 86% and 90% amino acid (aa) sequence identity with mouse and rat Caspase-3, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-03

Question

I see that the anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-09

Question

We have observed staining in human lymph. Any tips? Is anti-Human Caspase-3 DyLight® 550 conjugated antibody supposed to stain lymph positively?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

Based on literature lymph does express CASP3. Based on Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-11-28

Question

We want to test anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550 on human t-cell for research purposes, then I may be interested in using anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

The products we sell, including anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-29

Question

Does anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550 work for Flow Cytometry with t-cell?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

According to the expression profile of t-cell, CASP3 is highly expressed in t-cell. So, it is likely that anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550 will work for Flow Cytometry with t-cell.

Boster Scientific Support

Answered: 2019-09-04

Question

Do you have a BSA free version of anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550 available?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-17

Question

I have a question about product A00334-Dyl550, anti-Human Caspase-3 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00334-Dyl550 anti-Human Caspase-3 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-18

Question

Does A00334-Dyl550 anti-Human Caspase-3 DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Wu

Verified customer

Asked: 2019-05-17

Answer

It shows on the product datasheet, A00334-Dyl550 anti-Human Caspase-3 DyLight® 550 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-17

Question

Is a blocking peptide available for product anti-Human Caspase-3 DyLight® 550 conjugated antibody (A00334-Dyl550)?

Verified Customer

Verified customer

Asked: 2018-09-12

Answer

We do provide the blocking peptide for product anti-Human Caspase-3 DyLight® 550 conjugated antibody (A00334-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-09-12

Question

Our team were satisfied with the WB result of your anti-Human Caspase-3 DyLight® 550 conjugated antibody. However we have seen positive staining in t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-11-30

Answer

Based on literature, t-cell does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-11-30

Question

I was wanting to use your anti-Human Caspase-3 DyLight® 550 conjugated antibody for Flow Cytometry for human t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human t-cell identification?

Verified Customer

Verified customer

Asked: 2017-11-22

Answer

As indicated on the product datasheet, A00334-Dyl550 anti-Human Caspase-3 DyLight® 550 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-22

Question

We are currently using anti-Human Caspase-3 DyLight® 550 conjugated antibody A00334-Dyl550 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?

S. Miller

Verified customer

Asked: 2017-10-25

Answer

The anti-Human Caspase-3 DyLight® 550 conjugated antibody (A00334-Dyl550) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-25

Size

Total: $515

SKU:A00334-Dyl550

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A00334-Dyl550
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