Product Info Summary
SKU: | PA1490 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase 4/CASP4 Antibody
SKU/Catalog Number
PA1490
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase 4/CASP4 Antibody catalog # PA1490. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase 4/CASP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1490)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase 4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1490 is reactive to CASP4 in Human
Applications
PA1490 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
20 kDa
Calculated molecular weight
43.262kDa
Background of CASP4
Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons.The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Caspase 4 antibody, PA1490, Western blotting
All lanes: Anti Caspase 4 (PA1490) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: CEM Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 43KD
Observed bind size: 20KD
Click image to see more details
Anti-Caspase 4 antibody, PA1490, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CASP4 (Source: Uniprot.org, NCBI)
Gene Name
CASP4
Full Name
Caspase-4
Weight
43.262kDa
Superfamily
peptidase C14A family
Alternative Names
apoptotic cysteine protease Mih1/TX; CASP4; CASP-4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase4; Caspase-4; EC 3.4.22; EC 3.4.22.57; ICE(rel)II; ICE(rel)-II; ICEREL-II; ICH2; ICH-2; Mih1/TX; Protease ICH-2; Protease TX; TX CASP4 ICE(rel)II, ICEREL-II, ICH-2, Mih1, Mih1/TX, TX caspase 4 caspase-4|CASP-4|ICE and Ced-3 homolog 2|ICE(rel)-II|apoptotic cysteine protease Mih1/TX|caspase 4, apoptosis-related cysteine peptidase|caspase 4, apoptosis-related cysteine protease|caspase-4 isoform alpha|protease ICH-2|protease TX
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP4, check out the CASP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase 4/CASP4 Antibody (PA1490)
Hello CJ!
PA1490 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ji J, Yan X, Li Z, Lai Z, Liu J. Biomed Pharmacother. 2015 Feb;69:311-6. Doi: 10.1016/J.Biopha.2014.12.020. Epub 2014 Dec 19. Therapeutic Effects Of Intrathecal Versus Intravenous Monosialoganglioside Against Bupivacaine-Induced Spinal Neurotoxici...
Chen J, Wei H, Xie B, Wang B, Cheng J, Cheng J. Leuk Res. 2012 Dec;36(12):1526-35. Doi: 10.1016/J.Leukres.2012.08.018. Epub 2012 Sep 7. Endoplasmic Reticulum Stress Contributes To Arsenic Trioxide-Induced Apoptosis In Drug-Sensitive And -Resistant...
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Caspase 4/CASP4 Antibody
Question
We are currently using anti-Caspase 4/CASP4 antibody PA1490 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
L. Williams
Verified customer
Asked: 2015-08-19
Answer
The anti-Caspase 4/CASP4 antibody (PA1490) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-08-19