Anti-Caspase-8 CASP8 Antibody

Product Name

Anti-Caspase-8 CASP8 Antibody

View all Caspase-8 Antibodies

SKU/Catalog Number

A00042-4

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Caspase-8 CASP8 Antibody (Catalog # A00042-4). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Caspase-8 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Caspase-8 CASP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00042-4)

Host

Rabbit

Contents

Caspase-8 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A. The immunogen is located within the last 50 amino acids of Caspase-8.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.

Reactive Species

A00042-4 is reactive to CASP8 in Human, Mouse, Rat

Applications

A00042-4 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

55.391kDa

Background of Caspase-8

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 μg/mL.

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Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 μg/mL.

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Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 μg/mL.

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Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 μg/ml.

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Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 μg/ml.

Red: Caspase-8 Antibody (A00042-4)
Blue: DAPI staining

Gene/Protein Information For CASP8 (Source: Uniprot.org, NCBI)

Alternative Names

AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease

For more info on CASP8, check out the CASP8 Infographic

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In this infographic, you will see the following information for CASP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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