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Product Info Summary
| SKU: | PA1524 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-8(P10)/CASP8 Antibody
SKU/Catalog Number
PA1524
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-8 (P10)/CASP8 Antibody catalog # PA1524. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-8(P10)/CASP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1524)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-8(P10).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1524 is reactive to CASP8 in Human
Applications
PA1524 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
55.391kDa
Background of Caspase-8
Caspase 8 is a caspase protein. It most likely acts upon caspase 3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1beta converting enzyme (ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex (DISC). This gene contains at least 11 exons spanning approximately 30kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8.Caspase-8 deficiency in humans is compatible with normal development and shows that caspase-8 has a postnatal role in immune activation of naive lymphocytes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-Caspase-8(P10) antibody, PA1524, Western blotting
WB: HELA Cell Lysate
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Anti-Caspase-8(P10) antibody, PA1524, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CASP8 (Source: Uniprot.org, NCBI)
Gene Name
CASP8
Full Name
Caspase-8
Weight
55.391kDa
Superfamily
peptidase C14A family
Alternative Names
AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP8, check out the CASP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-8(P10)/CASP8 Antibody (PA1524)
Hello CJ!
PA1524 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou L,Yu W,Jayabalan DS,Niesvizky R,Jaffrey SR,Huang X,Xu G.Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function.Front Cell Dev Biol.2020 Dec 17;8:605989.doi:10.3389/f
Species: Human
PA1524 usage in article: APP:WB, SAMPLE:HEK293 CELL AND HELA CELL, DILUTION:NA
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol
Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL
Inhibitory activity of apogossypol in human prostate cancerin vitroandin vivo
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (?)-gossypol
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL
Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.
Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Caspase-8(P10)/CASP8 Antibody
Question
We were satisfied with the WB result of your anti-Caspase-8(P10)/CASP8 antibody. However we have been able to see positive staining in t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
From what I have seen in literature, t-cell does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-Caspase-8(P10)/CASP8 antibody PA1524 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The anti-Caspase-8(P10)/CASP8 antibody (PA1524) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-24
Question
My lab would like using your anti-Caspase-8(P10)/CASP8 antibody for syncytiotrophoblast cell differentiation involved in labyrinthine layer development studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
I appreciate your inquiry. This PA1524 anti-Caspase-8(P10)/CASP8 antibody is tested on hela cell lysate, mammary cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-14
Question
We have observed staining in human t-cell. What should we do? Is anti-Caspase-8(P10)/CASP8 antibody supposed to stain t-cell positively?
M. Carter
Verified customer
Asked: 2017-06-21
Answer
According to literature t-cell does express CASP8. According to Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2017-06-21
Question
We ordered your anti-Caspase-8(P10)/CASP8 antibody for WB on leukocyte in the past. I am using human, and We want to use the antibody for IHC next. My question regards examining leukocyte as well as b-cell thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
B. Gonzalez
Verified customer
Asked: 2013-12-19
Answer
I looked at the website and datasheets of our anti-Caspase-8(P10)/CASP8 antibody and it appears that PA1524 has been validated on human in both WB and IHC. Thus PA1524 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-12-19
