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Product Info Summary
| SKU: | M00042 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00042
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody catalog # M00042. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00042)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EEG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caspase-8
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00042 is reactive to CASP8 in Human
Applications
M00042 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
83, 88 kDa
Calculated molecular weight
55.391kDa
Background of Caspase-8
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Caspase-8 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CASP8 (Source: Uniprot.org, NCBI)
Gene Name
CASP8
Full Name
Caspase-8
Weight
55.391kDa
Superfamily
peptidase C14A family
Alternative Names
AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP8, check out the CASP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody (M00042)
Hello CJ!
M00042 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol
Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody
Question
We want using your anti-Caspase-8 Rabbit Monoclonal antibody for regulation of tumor necrosis factor-mediated signaling pathway studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
We appreciate your inquiry. This M00042 anti-Caspase-8 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-27
Question
Do you have a BSA free version of anti-Caspase-8 Rabbit Monoclonal antibody M00042 available?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-8 Rabbit Monoclonal antibody M00042 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-Caspase-8 Rabbit Monoclonal antibody M00042 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The anti-Caspase-8 Rabbit Monoclonal antibody (M00042) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-16
Question
We have observed staining in human b-cell thymus. Do you have any suggestions? Is anti-Caspase-8 Rabbit Monoclonal antibody supposed to stain b-cell thymus positively?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Based on literature b-cell thymus does express CASP8. Based on Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2020-03-06
Question
My colleagues were well pleased with the WB result of your anti-Caspase-8 Rabbit Monoclonal antibody. However we have been able to see positive staining in t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Based on literature, t-cell does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2019-12-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-8 Rabbit Monoclonal antibody M00042. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-10
Question
Is this M00042 anti-Caspase-8 Rabbit Monoclonal antibody reactive to the isotypes of CASP8?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
The immunogen of M00042 anti-Caspase-8 Rabbit Monoclonal antibody is A synthesized peptide derived from human Caspase-8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-11
Question
My question regarding product M00042, anti-Caspase-8 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00042 anti-Caspase-8 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-12
Question
I see that the anti-Caspase-8 Rabbit Monoclonal antibody M00042 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-03
Question
Will M00042 anti-Caspase-8 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
You can see on the product datasheet, M00042 anti-Caspase-8 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-28
Question
I was wanting to use your anti-Caspase-8 Rabbit Monoclonal antibody for WB for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
You can see on the product datasheet, M00042 anti-Caspase-8 Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-05
Question
Does anti-Caspase-8 Rabbit Monoclonal antibody M00042 work for WB with t-cell?
W. Bhatt
Verified customer
Asked: 2018-09-17
Answer
According to the expression profile of t-cell, CASP8 is highly expressed in t-cell. So, it is likely that anti-Caspase-8 Rabbit Monoclonal antibody M00042 will work for WB with t-cell.
Boster Scientific Support
Answered: 2018-09-17
Question
Is a blocking peptide available for product anti-Caspase-8 Rabbit Monoclonal antibody (M00042)?
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
We do provide the blocking peptide for product anti-Caspase-8 Rabbit Monoclonal antibody (M00042). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-06
Question
Here is the WB image, lot number and protocol we used for t-cell using anti-Caspase-8 Rabbit Monoclonal antibody M00042. Please let me know if you require anything else.
A. Patel
Verified customer
Asked: 2017-08-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-24
Question
I am interested in to test anti-Caspase-8 Rabbit Monoclonal antibody M00042 on human t-cell for research purposes, then I may be interested in using anti-Caspase-8 Rabbit Monoclonal antibody M00042 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Jones
Verified customer
Asked: 2015-10-28
Answer
The products we sell, including anti-Caspase-8 Rabbit Monoclonal antibody M00042, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-28
