Caspase-9 Antibodies

AND CASP9 ELISA kits, Caspase-9 Proteins

Many biological assays use antibodies to detect Caspase-9, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Caspase-9 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Caspase-9 antibodies...

We validate the specificity of these antibodies to Caspase-9 by testing them on tissues known to express CASP9 positively and negatively. Browse below to find the CASP9 antibody that suites your experiment. We have 21 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Caspase-9? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CASP9 Western Blot Guide

CASP9 Infographic by Boster Bio

All CASP9 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-Caspase-9/Casp9 Antibody Picoband®

32 Citations

IF analysis of Caspase-9/Casp9 using anti-Caspase-9/Casp9 antibody (A00080-7)

Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9/Casp9 antibody (A00080-7)

IHC analysis of Caspase-9/Casp9 using anti-Caspase-9/Casp9 antibody (A00080-7)

Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A00080-7
100 μg/vial

Anti-Caspase-9/CASP9 Antibody Picoband®

15 Citations

Western blot analysis of Caspase-9/CASP9 using anti-Caspase-9/CASP9 antibody (A00080-8)

Flow Cytometry analysis of Caco-2 cells using anti-Caspase-9/CASP9 antibody (A00080-8)

Flow Cytometry analysis of K562 cells using anti-Caspase-9/CASP9 antibody (A00080-8)

Human

ELISA, Flow Cytometry, WB

$370
Cat # A00080-8
100 μg/vial

Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody

14 Citations

Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-9 Antibody

Immunofluorescent analysis of HeLa cells treated with staurosporine, using Caspase-9 Antibody

Western blot analysis of Caspase-9 using anti-Caspase-9 antibody (M00080)

Human, Mouse

IP, IF, IHC, ICC, WB

$370
Cat # M00080
100 μl

Anti-Caspase-9 p35/Casp9 Antibody Picoband®

6 Citations

Western blot analysis of Caspase-9 p35/Casp9 using anti-Caspase-9 p35/Casp9 antibody (A00080-4)

Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9 p35/Casp9 antibody (A00080-4)

Mouse, Rat

ELISA, Flow Cytometry, WB

$370
Cat # A00080-4
100 μg/vial

Anti-Caspase 9/CASP9 Antibody Picoband®

1 Citations

Anti-Caspase-9 antibody, PA1595, Western blottingAll lanes: Anti Caspase-9 (PA1595) at 0

PPM-18 triggers apoptotic cell death in bladder cancer cells

Human

IHC, WB

$370
Cat # PA1595
100 μg/vial

Anti-Caspase-9/CASP9 Antibody Picoband®

1 Citations

Western blot analysis of Caspase-9/CASP9 using anti-Caspase-9/CASP9 antibody (A00080-5)

IHC analysis of Caspase-9/CASP9 using anti-Caspase-9/CASP9 antibody (A00080-5)

IF analysis of Caspase-9/CASP9 using anti-Caspase-9/CASP9 antibody (A00080-5)

Human

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A00080-5
100 μg/vial

Anti-Caspase-9/CASP9 Antibody Picoband®

Western blot analysis of Caspase-9/CASP9 using anti-Caspase-9/CASP9 antibody (A00080-6)

IHC analysis of Caspase-9/CASP9 using anti-Caspase-9/CASP9 antibody (A00080-6)

Human

ELISA, Flow Cytometry, IHC, WB

$370
Cat # A00080-6
100 μg/vial

Anti-pro Caspase 9 CASP9 Rabbit Monoclonal Antibody

7 Citations

All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature

Western blot analysis of pro Caspase 9 expression in Jurkat cell lysate

Human

Flow Cytometry, IHC, WB

$370
Cat # M00080-1
100 μl

Anti-Zebrafish Caspase 9/CASP9 Antibody Picoband®

Western blot analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (AZF1QSB1)

IHC analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (AZF1QSB1)

Zebrafish

IHC, WB

$470
Cat # AZF1QSB1
100 μg/vial

Anti-cleaved Caspase-9 p35 CASP9 Rabbit Monoclonal Antibody

7 Citations

Western blot analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (M00080-2)

Human, Mouse

IP, WB

$370
Cat # M00080-2
100 μl

Anti-Caspase-9/CASP9 Antibody Picoband®

2 Citations

Anti-Caspase-9 Picoband antibody, PB9332, Western blottingAll lanes: Anti Caspase-9 (PB9332) at 0

Human

$370
Cat # PB9332
100 μg/vial

Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature

Immunofluorescent analysis using the Antibody at 1:50 dilution

Human

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M00080-3
100 μl

Anti-Caspase-9 CASP9 Antibody

Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane

Western Blot Validation in Mouse Cell Line
Loading: 15 μg of 3T3/NIH cell lysate

Human, Mouse

ELISA, IP, IF, ICC, WB

$481
Cat # A00080-1
0.1 mg

Anti-Caspase 9 CASP9 Monoclonal Antibody

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000

Western blot analysis of lysates from 1) Hela, 2) Jurkat, 3)3T3 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue

Chicken, Human, Mouse, Rat

IP, IF, IHC, WB

$380
Cat # M00080-5
100ul

Anti-Caspase-9 Rabbit Monoclonal Antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Caspase-9 antibody

Human, Mouse, Rat

ELISA, IF, WB

$146
Cat # M00080-6
20μL, 40μL, 100μL, 200μL

Caspase 9 (CASP9) (NM_001229) Human Over-expression Lysate

Caspase 9 (CASP9) Human Over-expression Lysates NM_001229

Human

$628
Cat # LS000842
20 µg, 100 µg

Caspase 9 (Phospho-Ser196) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from 293 cells treated with Calyculin 50nM 30

Human

ELISA

$540
Cat # EKC2226
1 kit, containing two 96-well plates and all necessary reagents

Caspase 9 (Phospho-Ser144) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from K562 cells

Human

ELISA

$540
Cat # EKC2225
1 kit, containing two 96-well plates and all necessary reagents

Caspase 9 (Phospho-Tyr153) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from COLO205 cells

Human

ELISA

$540
Cat # EKC2224
1 kit, containing two 96-well plates and all necessary reagents

Caspase 9 (Phospho-Thr125) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells treated with TNF 20ng/ml 5

Human, Mouse, Rat

ELISA

$540
Cat # EKC1977
1 kit, containing two 96-well plates and all necessary reagents

Caspase 9 Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1089
1 kit, containing one 96-well plate and all necessary reagents

CASP9 Overview

Facts about Caspase-9.

CASP9 3D structure. Source: alphafold

Caspase-9 (CASP9)

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3.

Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent method. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP).

Gene Information

Human
Gene Name:	CASP9
Uniprot:	P55211
Entrez:	842

Family

Belongs to:

peptidase C14A family

Alternative Names

APAF-3APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase9; Caspase-9; EC 3.4.22.62; ICE-LAP6CASPASE-9c; ICE-like apoptotic protease 6; MCH6apoptosis-related cysteine protease

Molecular Weight

Mass (kDA):
46.281 kDA

Genomic Context


Human

Location:	1p36.21
Sequence:	1; NC_000001.11 (15491401..15524912, complement)

Tissue Specificity

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

Diseases

Malignant Neoplasms
Neoplasms
Carcinoma
Leukemia
Mammary Neoplasms
Malignant Paraganglionic Neoplasm
Malignant Neoplasm Of Breast
Lymphoma
Malignant Tumor Of Colon
Adenocarcinoma
Neuroblastoma
Ischemia
Colonic Neoplasms

Lung Neoplasms
Inflammation
Liver Carcinoma
Prostatic Neoplasms
Malignant Neoplasm Of Lung
Melanoma
Malignant Neoplasm Of Prostate

Interacting Proteins

APAF1
BIRC2
BIRC3
BIRC5
BIRC7

CASP3
DCC
ILK
XIAP

Pathways

Cell Death
Induction Of Apoptosis
Cell Cycle
Programmed Cell Death
Cell Proliferation
Pathogenesis
Release Of Cytochrome C From Mitochondria
Cell Growth
Apoptotic Process
Localization
Cell Cycle Arrest
Proteolysis
Sensitization

Secretion
Mitochondrial Depolarization
Methylation
Immune Response
Cell Killing
Neuroprotection
Translation

PTMs

Cleavage
Phosphorylation
Methylation
Dephosphorylation
Acetylation
Ubiquitination
Reduction
Oxidation
Nitrosylation
Demethylation

Phosphorothioation
Myristoylation
Nitration
Glycosylation
Prenylation
Ribosylation
Biotinylation
Sumoylation
Glutathionylation
Acylation

Research Areas

Alzheimers Research
Apoptosis
Cancer
Caspases
Cell Biology

Neurodegeneration

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CASP9

References

PMID: 8663294 by Duan H., et al. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.

PMID: 8900201 by Srinivasula S.M., et al. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32.

37 Publications of Boster Caspase-9 Antibodies & ELISA Kits

PubMed ID: 10.3892/or.2011.1163, GROβ and its downstream effector EGR1 regulate cisplatin-induced apoptosis in W...
PubMed ID: 10.3892/ol.2013.1449, Effects of matrine on the proliferation of HT29 human colon cancer cells and its...
PubMed ID: 10.3892/mmr.2014.2859, HIF‑1 signaling pathway involving iNOS, COX‑2 and caspase‑9 mediates the n...
PubMed ID: 10.3892/ijo.2015.3039, Autophagy is involved in recombinant Newcastle disease virus (rL-RVG)-induced ce...
PubMed ID: 10.3389/fphar.2021.609702, Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite De...
PubMed ID: 10.1248/bpb.b13-00794, Involvement of substance P/neurokinin-1 receptor in the analgesic and anticancer...
PubMed ID: 10.1186/s12985-017-0852-z, Recombinant Newcastle disease virus rL-RVG enhances the apoptosis and inhibits t...
PubMed ID: 10.1186/1556-276X-9-319, In vivo and in vitro evaluation of the cytotoxic effects of Photosan-loaded holl...
PubMed ID: 10.1111/dth.14720, CD4+T cells apoptosis and conversion of Th1/Th2‐type cytokines promote the pro...
PubMed ID: 10.1080/15376516.2017.1333553, Rotenone induces nephrotoxicity in rats: oxidative damage and apoptosis
PubMed ID: 10.1039/C5TB00514K, Design of magnetic nanoparticles for hepatocellular carcinoma treatment using th...
PubMed ID: 10.1016/j.ijpharm.2016.08.037, Vitamin E TPGS modified liposomes enhance cellular uptake and targeted delivery ...
PubMed ID: 10.1016/j.bbrc.2005.03.182, Inhibition of the PI3K pathway sensitizes fludarabine-induced apoptosis in human...
PubMed ID: 10.1002/ptr.5241, Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondr...
PubMed ID: -, Xuening Pang,Xiang Gao,Feng Liu,Yuhuan Jiang,Mingji Wang,Qun Li,Zichao Li,Xantho...
PubMed ID: 33654381, Xu Y,Chen W,Chen Z,Huang M,Yang F,Zhang Y.Mechanism of Action of Xiaoyao San in ...
PubMed ID: 32802118, Li QY,Hou CZ,Yang LP,Chu XL,Wang Y,Zhang P,Zhao Y. Study on the Mechanism of Gin...
PubMed ID: 33284899, Yue W,Cunlin G,Lu H,Yuanqing Z,Yanjun T,Qiong W. Neuroprotective effect of inter...
PubMed ID: 27915342, In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of t...
PubMed ID: 28197193, Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apo...
PubMed ID: 25024681, In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded holl...

*Showing only the more recent 20. More publications can be found for each product on its corresponding product page