Skip to the beginning of the images gallery

CASP9 antibody (A00080-8) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 15 Publications

a00080 8 casp9 primary antibodies fcm testing 2

Flow Cytometry analysis of Caco-2 cells using anti-Caspase-9/CASP9 antibody (A00080-8).
Overlay histogram showing Caco-2 cells stained with A00080-8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9/CASP9 Antibody (A00080-8, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-Caspase-9/CASP9 Antibody Picoband®

CASP9 antibody

15 Publications

Reactive Species	Host	Application
Human	Rabbit	ELISA, Flow Cytometry, WB

Tested Applications

CASP9 antibody was positively detected by WB in:

human Jurkat whole cell, human Hela whole cell, human HCCT tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CASP9 antibody was positively detected by FC in:

Caco-2 cell, K562 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

ELISA

Human

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-Caspase-9/CASP9 Antibody Picoband®
View all CASP9 Antibodies

Form

Lyophilized

Uniprot

P55211

Gene ID

842

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Caspase-9/CASP9 recombinant protein (Position: D53-N268).

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

46 kDa

Calculated Molecular Weight

46.3 kDa

Background of CASP9

CASP9 is also known as MCH6 or APAF3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Cite This Product

Anti-Caspase-9/CASP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00080-8)

Protocols

Western Blot protocol for CASP9 antibody

Review Protocol

Flow Cytometry (FC) protocol for CASP9 antibody

Review Protocol

Publications

Polyphenols From Morchella sextelata Induce Apoptosis of Colorectal Cancer Cells Through ROS-Mediated Endogenous Mitochondrial Apoptosis Pathway In Vitro

Published: 2025-01-16

Journal: JOURNAL OF FOOD BIOCHEMISTRY

Application: WB

Impact Factor: 4.20

Species: Human

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-Caspase-9/CASP9 Antibody Picoband®

CASP9 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Polyphenols From Morchella sextelata Induce Apoptosis of Colorectal Cancer Cells Through ROS-Mediated Endogenous Mitochondrial Apoptosis Pathway In Vitro

The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways

Sodium tanshinone IIA sulfonate protects ARPE-19 cells against oxidative stress by inhibiting autophagy and apoptosis

Recombinant Newcastle disease virus rL-RVG enhances the apoptosis and inhibits the migration of A549 lung adenocarcinoma cells via regulating alpha 7 nicotinic acetylcholine receptors in vitro

Rotenone induces nephrotoxicity in rats: oxidative damage and apoptosis

Vitamin E TPGS modified liposomes enhance cellular uptake and targeted delivery of luteolin: An in vivo/in vitro evaluation

Autophagy is involved in recombinant Newcastle disease virus (rL-RVG)-induced cell death of stomach adenocarcinoma cells in vitro

Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells

In vivo and in vitro evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer

Involvement of substance P/neurokinin-1 receptor in the analgesic and anticancer activities of minimally toxic fraction from the traditional Chinese medicine Liu-Shen-Wan in vitro

Effects of matrine on the proliferation of HT29 human colon cancer cells and its antitumor mechanism

Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs

GROβ and its downstream effector EGR1 regulate cisplatin-induced apoptosis in WHCO1 cells

Inhibition of the PI3K pathway sensitizes fludarabine-induced apoptosis in human leukemic cells through an inactivation of MAPK-dependent pathway

Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells

Review

Have you used Anti-Caspase-9/CASP9 Antibody Picoband®?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality