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Product Info Summary
| SKU: | M01617 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Catenin alpha 1 CTNNA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01617
BM4486 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Catenin alpha 1 CTNNA1 Rabbit Monoclonal Antibody catalog # M01617. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Catenin alpha 1 CTNNA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01617)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FBO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Catenin alpha 1
Reactive Species
M01617 is reactive to CTNNA1 in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
100.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01617 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: HeLa cell
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of CTNNA1 using anti-CTNNA1 antibody (M01617).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNA1 antigen affinity purified monoclonal antibody (M01617) at 1:10000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 100 kDa. The expected band size for CTNNA1 is at 100 kDa.
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Immunohistochemical analysis of paraffin-embedded human lung cancer, using Catenin alpha 1 Antibody.
Specific Publications For Anti-Catenin alpha 1 CTNNA1 Rabbit Monoclonal Antibody (M01617)
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Customer Q&As
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2 Customer Q&As for Anti-Catenin alpha 1 CTNNA1 Rabbit Monoclonal Antibody
Question
Will PB9137 and M01617 react with CTNNA2 and its isoforms?
Verified customer
Asked: 2019-11-20
Answer
Our lab hasn't experimentally validate the Anti-Alpha 1 Catenin/CTNNA1 Antibody Picoband™ (PB9137) and Anti-Catenin Alpha 1 CTNNA1 Rabbit Monoclonal Antibody (M01617) cross reactivity with CTNNA2 and its isoforms. Sequence homology with CTNNA2 and its isoforms is low.
Boster Scientific Support
Answered: 2019-11-20
Question
Will M01617 react with CTNNA2 and its isoforms?
Verified customer
Asked: 2019-11-20
Answer
Our lab hasn't experimentally validate the Anti-Catenin Alpha 1 CTNNA1 Rabbit Monoclonal Antibody (M01617) cross reactivity with CTNNA2 and its isoforms. Sequence homology with CTNNA2 and its isoforms is low.
Boster Scientific Support
Answered: 2019-11-20
