Anti-Catenin, beta (p120) CTNNB1 Monoclonal Antibody

Product Name

Anti-Catenin, beta (p120) CTNNB1 Monoclonal Antibody

View all beta-Catenin Antibodies

SKU/Catalog Number

M00004

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Catenin, beta (p120) CTNNB1 Monoclonal Antibody (Catalog # M00004). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Cow, Dog, Chicken.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Catenin, beta (p120) CTNNB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00004)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 15B8

Isotype

IgG1, kappa

Immunogen

Recombinant chicken β-catenin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00004 is reactive to CTNNB1 in Chicken, Cow, Dog, Human, Mouse, Rat

Applications

M00004 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

85.497kDa

Background of beta-Catenin

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human tonsil stained with Anti-Beta-Catenin (p120) Monoclonal Antibody (15B8).

Gene/Protein Information For CTNNB1 (Source: Uniprot.org, NCBI)

Alternative Names

bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923 CTNNB1 CTNNB, EVR7, MRD19, NEDSDV, armadillo catenin beta 1 catenin beta-1|catenin (cadherin-associated protein), beta 1, 88kDa

For more info on CTNNB1, check out the CTNNB1 Infographic

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In this infographic, you will see the following information for CTNNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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