Anti-Cathepsin LVKH CTSL Antibody

Product Name

Anti-Cathepsin LVKH CTSL Antibody

View all Cathepsin L Antibodies

SKU/Catalog Number

A01589-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Cathepsin LVKH CTSL Antibody catalog # A01589-1. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cathepsin LVKH CTSL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01589-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human p47-phox around the phosphorylation site of S370.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01589-1 is reactive to CTSL in Human, Mouse, Rat

Applications

A01589-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

37.564kDa

Background of Cathepsin L

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (). CLDN1 acts as a coreceptor for HCV entry into hepatic cells.

Julie Dufresne and Daniel G. Cyr, Biol Reprod, May 2007; 76: 825 - 832.
Evemie Dub¨¦, Biol Reprod, Apr 2007; 10.1095/biolreprod.106.059246.
Michele A. Battle, PNAS, May 2006; 103: 8419 - 8424.
Edmund C. Chang, Endocrinology, Oct 2006; 147: 4831 - 4842.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:5,000
ICC: 1:50-1:100
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of CTSL using Anti-Cathepsin LVKH CTSL Antibody (A01589-1).

ICC staining Cathepsin L/V/K/H in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-Cathepsin LVKH CTSL Antibody A01589-1

Western blot analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1

Gene/Protein Information For CTSL (Source: Uniprot.org, NCBI)

Alternative Names

Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP CTSL CATL1, MEP, CTSL cathepsin L procathepsin L|cathepsin L1|major excreted protein

For more info on CTSL, check out the CTSL Infographic

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In this infographic, you will see the following information for CTSL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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