Click image to see more details
Product Info Summary
| SKU: | A01683-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD146 MCAM Antibody
View all MCAM/CD146 Antibodies
SKU/Catalog Number
A01683-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD146 MCAM Antibody catalog # A01683-1. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD146 MCAM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01683-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PAHO protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01683-1 is reactive to MCAM in Human, Mouse, Rat
Applications
A01683-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
71.607kDa
Background of MCAM/CD146
Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development By similarity. Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.
Lucka L., FEBS Lett. 454:341-344(1999).
Seppala R., Am. J. Hum. Genet. 64:1563-1569(1999).
Huizing M., Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of MCAM using Anti-CD146 MCAM Antibody (A01683-1).
ICC staining CD146 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Click image to see more details
Figure 1. Western blotting validation for Anti-CD146 MCAM Antibody A01683-1
Western blot analysis of CD146 on SiHa cell using anti-CD146 antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MCAM (Source: Uniprot.org, NCBI)
Gene Name
MCAM
Full Name
Cell surface glycoprotein MUC18
Weight
71.607kDa
Alternative Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin MCAM CD146, HEMCAM, METCAM, MUC18, MelCAM melanoma cell adhesion molecule cell surface glycoprotein MUC18|Gicerin|S-endo 1 endothelial-associated antigen|cell surface glycoprotein P1H12|melanoma adhesion molecule|melanoma-associated antigen A32|melanoma-associated antigen MUC18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCAM, check out the MCAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD146 MCAM Antibody (A01683-1)
Hello CJ!
No publications found for A01683-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD146 MCAM Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-CD146 MCAM Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
