Anti-CD22 / BL-CAM Monoclonal Antibody

Product Name

Anti-CD22 / BL-CAM Monoclonal Antibody

View all Siglec-2/CD22 Antibodies

SKU/Catalog Number

M01572

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD22 / BL-CAM Monoclonal Antibody (Catalog # M01572). Tested in ELISA, Flow Cytometry, WB, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD22 / BL-CAM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01572)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: BLCAM/1795

Isotype

IgG1, kappa

Immunogen

Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01572 is reactive to CD22 in Human

Applications

M01572 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

95.348kDa

Background of Siglec-2/CD22

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Spleen stained with Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).

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Western Blot Analysis of Raji cell lysate using Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).

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Western Blot analysis of Raji and Ramos cell lysates using Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).

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Flow Cytometric analysis of paraformaldehyde-fixed MOLT4 cells. Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Gene/Protein Information For CD22 (Source: Uniprot.org, NCBI)

Alternative Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 antigen|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2

For more info on CD22, check out the CD22 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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