Product Info Summary
SKU: | M01572 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CD22 / BL-CAM Monoclonal Antibody
View all Siglec-2/CD22 Antibodies
SKU/Catalog Number
M01572
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD22 / BL-CAM Monoclonal Antibody (Catalog # M01572). Tested in ELISA, Flow Cytometry, WB, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD22 / BL-CAM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01572)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: BLCAM/1795
Isotype
IgG1, kappa
Immunogen
Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01572 is reactive to CD22 in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
95348 MW
Background of Siglec-2/CD22
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01572 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Spleen stained with Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).
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Western Blot Analysis of Raji cell lysate using Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).
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Western Blot analysis of Raji and Ramos cell lysates using Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).
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Flow Cytometric analysis of paraformaldehyde-fixed MOLT4 cells. Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Anti-CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Protein Target Info & Infographic
Gene/Protein Information For CD22 (Source: Uniprot.org, NCBI)
Gene Name
CD22
Full Name
B-cell receptor CD22
Weight
95348 MW
Superfamily
immunoglobulin superfamily
Alternative Names
B-cell receptor CD22;B-lymphocyte cell adhesion molecule;BL-CAM;Sialic acid-binding Ig-like lectin 2;Siglec-2;T-cell surface antigen Leu-14;CD22;CD22;SIGLEC2; CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 |T-cell surface Leu-14|sialic acid-binding Ig-like lectin 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD22, check out the CD22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD22 / BL-CAM Monoclonal Antibody (M01572)
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Customer Q&As
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3 Customer Q&As for Anti-CD22 / BL-CAM Monoclonal Antibody
Question
My colleagues were content with the WB result of your anti-CD22 / BL-CAM Monoclonal antibody. However we have been able to see positive staining in tonsil cell membrane using this antibody. Is that expected? Could you tell me where is CD22 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-05
Answer
According to literature, tonsil does express CD22. Generally CD22 expresses in cell membrane. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119
Boster Scientific Support
Answered: 2018-09-05
Question
We have seen staining in human corpus callosum. Are there any suggestions? Is anti-CD22 / BL-CAM Monoclonal antibody supposed to stain corpus callosum positively?
K. Bhatt
Verified customer
Asked: 2013-02-08
Answer
According to literature corpus callosum does express CD22. According to Uniprot.org, CD22 is expressed in corpus callosum, tonsil, spleen, among other tissues. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119
Boster Scientific Support
Answered: 2013-02-08
Question
We are currently using anti-CD22 / BL-CAM Monoclonal antibody M01572 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
J. Krishna
Verified customer
Asked: 2013-01-09
Answer
The anti-CD22 / BL-CAM Monoclonal antibody (M01572) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-09