Product Info Summary
SKU: | A01114 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD40L CD40LG Antibody
View all CD40 Ligand/TNFSF5 Antibodies
SKU/Catalog Number
A01114
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD40L CD40LG Antibody catalog # A01114. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40L CD40LG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01114)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Strad
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01114 is reactive to CD40LG in Human, Mouse
Applications
A01114 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
29.274kDa
Background of CD40 Ligand/TNFSF5
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Scanlan M.J., Cancer Immun. 1:4-4(2001).
Ota T., Nat. Genet. 36:40-45(2004).
Baas A.F., EMBO J. 22:3062-3072(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of CD40LG using Anti-CD40L CD40LG Antibody (A01114).
ICC staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-CD40L CD40LG Antibody A01114
Western blot analysis of CD40L on different cells lysates using anti-CD40L antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Mouse liverElectrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CD40LG (Source: Uniprot.org, NCBI)
Gene Name
CD40LG
Full Name
CD40 ligand
Weight
29.274kDa
Superfamily
tumor necrosis factor family
Alternative Names
CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5 CD40LG CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L CD40 ligand CD40 ligand|CD40 antigen ligand|CD40-L|T-B cell-activating molecule|T-cell antigen Gp39|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD40LG, check out the CD40LG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD40LG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD40L CD40LG Antibody (A01114)
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No publications found for A01114
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