Anti-CD43 (T-Cell Marker) SPN Monoclonal Antibody

Product Name

Anti-CD43 (T-Cell Marker) SPN Monoclonal Antibody

View all CD43/Sialophorin Antibodies

SKU/Catalog Number

M01296

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD43 (T-Cell Marker) SPN Monoclonal Antibody (Catalog # M01296). Tested in WB, Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD43 (T-Cell Marker) SPN Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01296)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPN/1094

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human SPN protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01296 is reactive to SPN in Human

Applications

M01296 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

40.322kDa

Background of CD43/Sialophorin

This monoclonal antibody recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Lymphoma stained with Anti-CD43 Mouse Monoclonal Antibody (SPN/1094).

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD43 Mouse Monoclonal Antibody (SPN/1094).

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Immunofluorescence analysis of K562 cells labeling CD43 with Anti-CD43 Mouse Monoclonal Antibody (SPN/1094) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot.

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Western Blot analysis of K562 cell lysate using Anti-CD43 Mouse Monoclonal Antibody (SPN/1094).

Gene/Protein Information For SPN (Source: Uniprot.org, NCBI)

Alternative Names

CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN SPN CD43, GALGP, GPL115, LSN sialophorin leukosialin|galactoglycoprotein|leukocyte sialoglycoprotein|sialophorin (gpL115, leukosialin, CD43)

For more info on SPN, check out the SPN Infographic

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In this infographic, you will see the following information for SPN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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