Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody

CD5 antibody

Boster Bio Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody (Catalog # M00480). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Horse, Pig, Rabbit, Cow, Rat, Guinea pig, Chicken.

Product Info Summary

SKU: M00480
Size: 100ug/vial
Reactive Species: Chicken, Cow, Guinea pig, Human, Monkey, Pig, Rabbit, Rat, Horse
Host: Mouse
Application: Flow Cytometry, IF, IHC

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Product Name

Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody

View all CD5 Antibodies

SKU/Catalog Number

M00480

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody (Catalog # M00480). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Horse, Pig, Rabbit, Cow, Rat, Guinea pig, Chicken.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00480)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: CD5/54/F6

Isotype

IgG1, kappa

Immunogen

Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00480 is reactive to CD5 in Chicken, Cow, Guinea pig, Human, Monkey, Pig, Rabbit, Rat, Horse

Applications

M00480 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

54.578kDa

Background of CD5

Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Reconstitute with 1ml water (15 minutes at room temperature).

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)

Gene Name

CD5

Full Name

T-cell surface glycoprotein CD5

Weight

54.578kDa

Alternative Names

CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 antigen (p56-62)|epididymis secretory sperm binding protein|lymphocyte antigen T1/Leu-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD5, check out the CD5 Infographic

CD5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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15 Customer Q&As for Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody

Question

Will anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 work for IHC with pancreas?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

According to the expression profile of pancreas, CD5 is highly expressed in pancreas. So, it is likely that anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 will work for IHC with pancreas.

Boster Scientific Support

Answered: 2020-03-12

Question

Does M00480 anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

It shows on the product datasheet, M00480 anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-29

Question

Is a blocking peptide available for product anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody (M00480)?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

We do provide the blocking peptide for product anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody (M00480). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-15

Question

My question regarding product M00480, anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00480 anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-07

Question

I was wanting to use your anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody for IHC for monkey pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for monkey pancreas identification?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

You can see on the product datasheet, M00480 anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody has been tested for IHC on bovine, chicken, equine, guinea pig, human, monkey, pig, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in monkey pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-17

Question

Is this M00480 anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody reactive to the isotypes of CD5?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

The immunogen of M00480 anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody is A synthetic peptide from the intracellular region of human CD5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-13

Question

I have attached the WB image, lot number and protocol we used for pancreas using anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-22

Question

Would anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 work on pig IHC with pancreas?

Verified Customer

Verified customer

Asked: 2018-06-18

Answer

Our lab technicians have not tested anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig pancreas in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-18

Question

Do you have a BSA free version of anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 available?

J. Edwards

Verified customer

Asked: 2018-06-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-06-15

Question

I am interested in to test anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 on monkey pancreas for research purposes, then I may be interested in using anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-04-04

Answer

The products we sell, including anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-04

Question

I see that the anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-02-02

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-02-02

Question

We are currently using anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480 for chicken tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says bovine, chicken, equine, guinea pig, human, monkey, pig, rabbit, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-15

Answer

The anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody (M00480) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-15

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody M00480. Let me know if you need anything else.

L. Yang

Verified customer

Asked: 2017-05-08

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-08

Question

My team were content with the WB result of your anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?

T. Walker

Verified customer

Asked: 2016-07-27

Answer

According to literature, leukemic t-cell does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-07-27

Question

We have been able to see staining in rabbit pancreas. Any tips? Is anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal antibody supposed to stain pancreas positively?

P. Anderson

Verified customer

Asked: 2016-04-08

Answer

According to literature pancreas does express CD5. According to Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-04-08

Order DetailsPrice
M00480-100ug

100 μg

$468

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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