Anti-CD79a Monoclonal Antibody

Product Name

Anti-CD79a Monoclonal Antibody

View all CD79A Antibodies

SKU/Catalog Number

M01047

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD79a Monoclonal Antibody (Catalog # M01047). Tested in WB, Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Pig, Cow, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD79a Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01047)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: HM57

Isotype

IgG1, kappa

Immunogen

A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01047 is reactive to CD79A in Cow, Human, Monkey, Mouse, Pig, Rat

Applications

M01047 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

25.038kDa

Background of CD79A

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD79a Monoclonal Antibody (HM57).

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Western Blot Analysis of Raji cell lysate using Anti-CD79a Monoclonal Antibody (HM57).

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Flow Cytometric Analysis of Raji cells using Anti-CD79a Monoclonal Antibody (HM57) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence analysis of PFA-fixed Raji cells labeling CD79a with Anti-CD79a Monoclonal Antibody (HM57) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Gene/Protein Information For CD79A (Source: Uniprot.org, NCBI)

Alternative Names

CD79A antigen (immunoglobulin-associated alpha); CD79a antigen; CD79a molecule, immunoglobulin-associated alpha; CD79A; IGAB-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; MB1; MB-1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein CD79A IGA, MB-1 CD79a molecule B-cell antigen receptor complex-associated protein alpha chain|CD79a antigen (immunoglobulin-associated alpha)|CD79a molecule, immunoglobulin-associated alpha|MB-1 membrane glycoprotein|ig-alpha|membrane-bound immunoglobulin-associated protein|surface IgM-associated protein

For more info on CD79A, check out the CD79A Infographic

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In this infographic, you will see the following information for CD79A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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