Anti-CD90 / Thy1 Rabbit Monoclonal Antibody

THY1 antibody

Boster Bio Anti-CD90 / Thy1 Rabbit Monoclonal Antibody catalog # M01818. Tested in WB, IHC applications. This antibody reacts with Human, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: M01818
Size: 100 μl
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD90 / Thy1 Rabbit Monoclonal Antibody

View all THY1 Antibodies

SKU/Catalog Number

M01818

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD90 / Thy1 Rabbit Monoclonal Antibody catalog # M01818. Tested in WB, IHC applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD90 / Thy1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01818)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CEB-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD90 / Thy1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01818 is reactive to THY1 in Human, Rat

Applications

M01818 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

17.935kDa

Background of THY1

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For THY1 (Source: Uniprot.org, NCBI)

Gene Name

THY1

Full Name

Thy-1 membrane glycoprotein

Weight

17.935kDa

Alternative Names

CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1 THY1 CD90, CDw90 Thy-1 cell surface antigen thy-1 membrane glycoprotein|Thy-1 T-cell antigen|thy-1 antigen

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on THY1, check out the THY1 Infographic

THY1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for THY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01818 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy

Differentiation of mesenchymal stem cell in the microenviroment of retinitis pigmentosa

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Li F, Xu Y, Xu X, Xu B, Zhao J, Zhang X. Mol Med Rep. 2014 Jul;10(1):322-8. Doi: 10.3892/Mmr.2014.2220. Epub 2014 May 8. Fms-Related Tyrosine Kinase??3 Ligand Promotes Proliferation Of Placenta Amnion And Chorion Mesenchymal Stem Cells In??vitro.

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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

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15 Customer Q&As for Anti-CD90 / Thy1 Rabbit Monoclonal Antibody

Question

Is this M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody reactive to the isotypes of THY1?

S. Singh

Verified customer

Asked: 2020-03-27

Answer

The immunogen of M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD90 / Thy1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-27

Question

Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-13

Question

Can you help my question with product M01818, anti-CD90 / Thy1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-18

Question

Will anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 work for WB with leukemic t-cell?

L. Zhao

Verified customer

Asked: 2019-09-26

Answer

According to the expression profile of leukemic t-cell, THY1 is highly expressed in leukemic t-cell. So, it is likely that anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 will work for WB with leukemic t-cell.

Boster Scientific Support

Answered: 2019-09-26

Question

I see that the anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 works with WB, what is the protocol used to produce the result images on the product page?

O. Huang

Verified customer

Asked: 2019-09-17

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-17

Question

We bought anti-CD90 / Thy1 Rabbit Monoclonal antibody for IHC on leukemic t-cell last year. I am using rat, and I plan to use the antibody for WB next. I am interested in examining leukemic t-cell as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

I viewed the website and datasheets of our anti-CD90 / Thy1 Rabbit Monoclonal antibody and I see that M01818 has been tested on rat in both IHC and WB. Thus M01818 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-11

Question

Is a blocking peptide available for product anti-CD90 / Thy1 Rabbit Monoclonal antibody (M01818)?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

We do provide the blocking peptide for product anti-CD90 / Thy1 Rabbit Monoclonal antibody (M01818). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-29

Question

I was wanting to use your anti-CD90 / Thy1 Rabbit Monoclonal antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

You can see on the product datasheet, M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-28

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-28

Question

We are currently using anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-02-25

Answer

The anti-CD90 / Thy1 Rabbit Monoclonal antibody (M01818) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-25

Question

My lab would like to test anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-01-15

Answer

The products we sell, including anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-01-15

Question

Our team were content with the WB result of your anti-CD90 / Thy1 Rabbit Monoclonal antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is THY1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-05-28

Answer

Based on literature, brain does express THY1. Generally THY1 expresses in cell membrane. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2018-05-28

Question

We have seen staining in rat leukemic t-cell. Do you have any suggestions? Is anti-CD90 / Thy1 Rabbit Monoclonal antibody supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2017-06-27

Answer

Based on literature leukemic t-cell does express THY1. Based on Uniprot.org, THY1 is expressed in anterior cingulate cortex, brain, amygdala, eye, liver, leukemic t-cell, among other tissues. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2017-06-27

Question

Is there a BSA free version of anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 available?

J. Miller

Verified customer

Asked: 2015-04-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-04-13

Question

Would M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Edwards

Verified customer

Asked: 2013-01-01

Answer

It shows on the product datasheet, M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-01-01

Order DetailsPrice
M01818

100μL

$370
M01818-30ul

30μL sample

$99
M01818-carrier-free

Carrier Free

$370

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M01818
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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