Product Info Summary
SKU: | A03905-2 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-CDC27 Antibody
SKU/Catalog Number
A03905-2
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-CDC27 Antibody (Catalog # A03905-2). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-CDC27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03905-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 415-445 of Human cdc27.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03905-2 is reactive to CDC27 in Human
Applications
A03905-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
91.867kDa
Background of CDC27
Cdc27 (also known as cell division cycle protein 27 homologue, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. Cdc27 is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Cdc27 has a nuclear localization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:40,000
IF Microscopy: User optimized
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CDC27 using anti-CDC27 antibody (A03905-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC27 antigen affinity purified polyclonal antibody (Catalog # A03905-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CDC27.
Protein Target Info & Infographic
Gene/Protein Information For CDC27 (Source: Uniprot.org, NCBI)
Gene Name
CDC27
Full Name
Cell division cycle protein 27 homolog
Weight
91.867kDa
Superfamily
APC3/CDC27 family
Alternative Names
ANAPC3CDC27 homolog; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC 3; APC3cell division cycle 27; CDC27Hs; cell division cycle 27 homolog (S. cerevisiae); cell division cycle protein 27 homolog; D0S1430E; D17S978E; HNUC; H-NUC; nuc2 homolog; NUC2 CDC27 ANAPC3, APC3Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, CDC27 cell division cycle 27 cell division cycle protein 27 homolog|D0S1430E, D17S978E|anaphase promoting complex subunit 3|anaphase-promoting complex, protein 3|cell division cycle 27 homolog|nuc2 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC27, check out the CDC27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDC27 Antibody (A03905-2)
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No publications found for A03905-2
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